中国听力语言康复科学杂志2025，Vol.23Issue(2)：221-224,4.DOI:10.3969/j.issn.1672-4933.2025.02.026
基于KLF3-AS1/miR-83-5p/TCF7L2轴的内耳干细胞定向神经分化作用机制
The Mechanism of Targeted Neural Differentiation of Inner Ear Stem Cells Based on Klf3-As1/Mir-83-5p/Tcf7l2 Axis
摘要
Abstract
Objective To explore the mechanism of targeted neural differentiation of inner ear stem cells based on KLF3-AS1/miR-83-5p/TCF7L2 axis.Methods Thirty healthy SD rats were selected and divided into 12 blank groups,9 protein culture groups and 9 protein culture+LY294002 groups.The neuronal axon length,apoptosis rate,cell activity and axon-related protein expression levels of KLF3-AS1/miR-83-5p/TCF7L2 were compared among the three groups.Results Compared with the blank group and the protein culture+LY294002 group,apoptosis rate,miR-83-5p protein expression,neuron axon length were lower in the protein culture group,and KLF3-AS1,cell activity and TCF7L2 protein expression were higher.The difference was statistically significant(P<0.05).Conclusion Downregulation of KLF3-AS1/miR-83-5p/TCF7L2 can promote the activity of inner ear stem cells,reduce apoptosis,and play an important role in targeted neural differentiation.关键词
转录因子7类似物2基因/Krüppel样因子3-反义转录物1/微小RNA-83-5p/内耳干细胞/定向神经分化Key words
Transcription factor 7 analogue 2 gene/Kruppel-like factor 3-antisense transcript 1/Microrna-83-5p/Inner ear stem cells/Directed neural differentiation分类
医药卫生引用本文复制引用
蒋迪,毛志强,傅明..基于KLF3-AS1/miR-83-5p/TCF7L2轴的内耳干细胞定向神经分化作用机制[J].中国听力语言康复科学杂志,2025,23(2):221-224,4.基金项目
广东省自然科学基金"miR-124上调BDNF-TrkB信号通路在内耳干细胞定向分化中的作用及机制研究"(2019A1515110158) （2019A1515110158）
广东省自然科学基金"CSF1/CCL22反馈环介导鼻咽癌细胞与TAMs相互作用促鼻咽癌转移的分子机制"(2021B1515140070) （2021B1515140070）
