中国现代医学杂志2025，Vol.35Issue(5)：24-31,8.DOI:10.3969/j.issn.1005-8982.2025.05.005
低氧条件下M2型巨噬细胞外泌体对骨髓间充质干细胞成骨分化的影响机制
Mechanism of M2 macrophage-derived exosomes on osteogenic differentiation of bone marrow mesenchymal stem cells under hypoxic conditions
摘要
Abstract
Objective To investigate the mechanism by which exosomes derived from M2 macrophages under hypoxic conditions regulate the osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs).Methods Murine macrophage line RAW264.7 and murine BMSC line CP-M131 were cultured to the third passage.Macrophages were polarized to M2 phenotype using interleukin-4.Exosomes from M2 macrophages cultured under normoxic(N-M2 exo)and hypoxic(H-M2 exo)conditions were extracted and characterized.CP-M131 cells were divided into PBS group,N-M2 exo group(treated with 90 ng/mL N-M2 exo),and H-M2 exo group(treated with 90 ng/mL H-M2 exo)for 72 hours.Exosome uptake by BMSCs was observed via immunofluorescence.Quantitative real-time PCR(qRT-PCR)and Western blotting were used to detect mRNA and protein expression of bone morphogenetic protein-2(BMP-2),Alkaline phosphatase(ALP),Runt-related transcription factor(Runx),extracellular signal-regulated kinase(ERK),and phosphorylated ERK(p-ERK).ALP staining was performed to assess osteogenic differentiation.Results M2 macrophage polarization and exosome isolation were successfully achieved.Compared to the N-M2 exo group,the H-M2 exo group exhibited significantly upregulated mRNA and protein expression of BMP-2,ALP,and Runx in CP-M131 cells(P＜0.05).ERK and p-ERK protein expression were also elevated in the H-M2 exo group(P＜0.05).ALP staining confirmed enhanced osteogenic differentiation in the H-M2 exo group.Conclusion Hypoxic M2 macrophage-derived exosomes promote osteogenic differentiation of BMSCs through ERK pathway activation.关键词
骨髓间充质干细胞/M2型巨噬细胞/外泌体/骨分化/氧条件Key words
bone marrow mesenchymal stem cell/M2 macrophages/exosomes/osteogenesis/hypoxia分类
医药卫生引用本文复制引用
吕梦姣,杨毅,田晓寒,廖礼彬,阿不都热西提·库提彼丁,王梦慈,周晓丹,袁孙强,白生宾..低氧条件下M2型巨噬细胞外泌体对骨髓间充质干细胞成骨分化的影响机制[J].中国现代医学杂志,2025,35(5):24-31,8.基金项目
国家自然科学基金(No:82160356,No:82160218) （No:82160356,No:82160218）
青年科技拔尖人才项目(No:2022TSYCCX0100) （No:2022TSYCCX0100）
省部共建中亚高发病成因与防治国家重点实验室项目(No:SKL-HIDCA-2024-GX1) （No:SKL-HIDCA-2024-GX1）
