中国现代医学杂志2025，Vol.35Issue(5)：32-38,7.DOI:10.3969/j.issn.1005-8982.2025.05.006
胶原蛋白海绵联合骨髓浓缩物复合物修复兔颅骨缺损的实验研究
Experimental study on collagen sponge combined with bone marrow concentrate composite for repairing rabbit skull defects
摘要
Abstract
Objective To investigate the efficacy of a composite material prepared from collagen sponge combined with bone marrow concentrate(BMC)in repairing rabbit skull defects.Methods Cranial defect models were established in 12 New Zealand rabbits.Bone marrow(5 mL)was aspirated from the bilateral femurs of the rabbits and centrifuged to obtain BMC,which was then mixed with collagen sponge to form a composite.Four full-thickness skull defects(8 mm in diameter)were created on both sides of the sagittal suture while preserving the intact dura mater.The rabbits were divided into four groups:blank control group(no implant),BMC group(BMC only),collagen group(collagen sponge only),and composite group(BMC-collagen composite).Four rabbits were euthanized at 4,8,and 12 weeks postoperatively,respectively,for skull defect specimen collection.Micro-CT scanning,hematoxylin-eosin(HE)staining,and Masson staining were performed to evaluate osteogenesis.Results Micro-CT scans at 4,8,and 12 weeks postoperatively demonstrated greater new bone formation in the composite group.Comparisons of bone mineral density(BMD)and bone volume/total volume(BV/TV)ratios among the four groups revealed:(1)Significant differences in BMD and BV/TV values across time points(P＜0.05);(2)Significant intergroup differences in BMD and BV/TV values(P＜0.05);(3)Significant differences in BMD trends among groups(P＜0.05),while no significant differences in BV/TV trends(P＞0.05).HE and Masson staining showed earlier and more mature new bone formation in the composite group compared to other groups.Conclusion The BMC-collagen composite significantly accelerates skull defect repair in rabbits and enhances bone regeneration,demonstrating potent osteogenic potential.关键词
骨缺损/胶原蛋白海绵/骨髓浓缩物/骨组织工程Key words
bone defect/collagen sponge/bone marrow concentrate/bone tissue engineering分类
临床医学引用本文复制引用
韩于辉,戚秀霞,汪世民,沈宇青,苏笑笑,郑先雨..胶原蛋白海绵联合骨髓浓缩物复合物修复兔颅骨缺损的实验研究[J].中国现代医学杂志,2025,35(5):32-38,7.基金项目
安徽省卫生健康科研项目(No:AHWJ2023A20179) （No:AHWJ2023A20179）
安徽省高等学校省级质量工程项目(No:2023jyxm0241) （No:2023jyxm0241）
安徽医科大学口腔医学院学科建设项目(No:2022xkfyhz08) （No:2022xkfyhz08）
