中国畜牧杂志2025，Vol.61Issue(3)：186-193,8.DOI:10.19556/j.0258-7033.20241022-01
视黄酸调控卵泡颗粒细胞雌激素合成及其改善后备母猪发情和繁殖力的应用
Application of Retinoic Acid in Regulating Estrogen Synthesis in Follicular Granulosa Cells and Improving Estrus Initiation and Fertility in Gilts
摘要
Abstract
This study aimed to investigate the effect of retinoic acid(RA),the major active metabolite of vitamin A(VA),on the expression of Cyp19a1,the rate-limiting enzyme for estrogen synthesis,as well as the role of VA in improving estrous initiation.Granulosa cells isolated from mouse and porcine ovaries were cultured in basal medium supplemented with 70 nmol/L or 7 μmol/L RA to assess Cyp19a1 expression and estrogen synthesis.Twenty-four 24-day-old female mice were randomly divided into four groups and intraperitoneally injected daily with 0,35,70,or 140 μg RA for 10 consecutive days to evaluate estrous cyclicity.Additionally,478 gilts were assigned to four groups and fed diets containing 0,12,500,25,000,or 50,000 IU VA daily for 70 days to monitor estrous behavior,with subsequent reproductive performance tracked in a subset of sows.Results demonstrated that exogenous RA significantly upregulated Cyp19a1 expression in both mouse and porcine granulosa cells(P<0.05)and enhanced estrogen synthesis in porcine granulosa cells(P<0.05)compared to controls.Intraperitoneal administration of 35 μg RA increased the estrous rate and synchronization in mice,while dietary supplementation with 25,000 IU vitamin A improved estrous rate and synchronization in gilts(P<0.05).These findings indicate that RA stimulates Cyp19a1 expression in follicular granulosa cells,thereby promoting estrogen synthesis and enhancing pubertal estrous initiation.Dietary VA supplementation can be used to improve estrous initiation and reproductive efficiency in gilts.关键词
视黄酸/卵泡颗粒细胞/雌激素/维生素A/发情:小鼠/母猪Key words
Retinoic acid/Granulosa cells/Estrogen/Vitamin A/Estrous/Mouse/Gilts分类
农业科技引用本文复制引用
孙果,田见晖,初美强,王福鹏,史志成,安磊,王书敏,程玲华,郭敏,李琴..视黄酸调控卵泡颗粒细胞雌激素合成及其改善后备母猪发情和繁殖力的应用[J].中国畜牧杂志,2025,61(3):186-193,8.基金项目
国家自然科学基金重点项目(31930103) （31930103）
"十四五"国家重点研发计划项目(2022YFD1300301) （2022YFD1300301）
宁波市重大专项(2021Z112) （2021Z112）
北京市家畜创新团队(BAIC05-2024) （BAIC05-2024）
