中国烟草科学2025，Vol.46Issue(1)：46-55,10.DOI:10.13496/j.issn.1007-5119.2025.01.007
两种促生菌对烟草生长和根际土壤细菌群落的影响
Effects of Two Growth-promoting Strains on Tobacco Growth and Bacterial Community in Rhizosphere Soil
摘要
Abstract
In order to explore the effects of two growth-promoting strain on tobacco growth and rhizosphere soil bacterial community,a pot experiment was conducted by using Yunyan 87 and set up with four groups:sterile water(cotrol),Piriformospora indica(PI),Bacillus subtilis(Tpb55),and P.indica+B.subtilis(PI+Tpb55).The growth index of tobacco plant,rhizosphere soil enzyme activity,bacterial community structure and function were analyzed.Results showed that compared to the control,both P.indica and B.subtilis Tpb55 promoted tobacco growth and increased dry or fresh matter accumulation,and PI+Tpb55 exhibited apparently higher effect than the single PI or Tpb55(p<0.05).PI,Tpb55,and PI+Tpb55 increased the activities of sucrase,urease,and acid phosphatase in tobacco rhizosphere soil,with the most significant effect(p<0.05)in PI+Tpb55.The above three treatments also increased the number,richness,and diversity of bacterial OTUs in tobacco rhizosphere soil,among which PI+Tpb55 exhibited significant effect,with Chao 1 index increased by 43.9%and Simpson index decreased by 10.1%.After PI+Tpb55 treatment,compared with the control,the relative abundance of Gemmatimonadota,Actinobacteria,and Acidobacteriota increased by 40%,20.6%,and 90%,respectively.The relative abundance of Gemmatimonas,Vicinamidobacteriaceae,and Haliangium increased by 35.5%,86.5%,and 135.7%,respectively,up to significant level(p<0.05).Functional prediction of bacterial community indicated that PI,Tpb55 and PI+Tpb55 increased the abundance of functional genes of bacterial community tricarboxylic acid cycle.In conclusion,both the strain P.indica and B.subtilis Tpb55 can promote tobacco growth and improve soil characteristics,with the optimal effect in the synergistic treatment.关键词
促生菌/根际土壤酶活性/细菌多样性/细菌群落功能/烟草Key words
growth-promoting strain/enzyme activity in rhizosphere soil/bacterial diversity/bacterial community function/tobacco分类
生物学引用本文复制引用
王睿,师晶,姚涛,孙文秀,曹毅,张成省..两种促生菌对烟草生长和根际土壤细菌群落的影响[J].中国烟草科学,2025,46(1):46-55,10.基金项目
中国烟草总公司重大科技项目[110202201004(JY-04)] （JY-04）
烟草行业烟草病虫害监测与综合治理重点实验室开放课题(KLTPMIMT2022-20) （KLTPMIMT2022-20）
