中国药房2025，Vol.36Issue(5)：552-557,6.DOI:10.6039/j.issn.1001-0408.2025.05.08
基于代谢组学研究柴胡皂苷C对急性肝损伤小鼠的保护作用
Study on the protective effect of saikosaponin C on acute liver injury in mice based on metabolomics
摘要
Abstract
OBJECTIVE To investigate the protective effect and mechanism of saikosaponin C(SSC)on acute liver injury(ALI)in mice induced by carbon tetrachloride(CCl4)based on serum metabolomics.METHODS Forty mice were divided into blank group(water),model group(water),positive control drug group(Biphenyl diester drop pills,150 mg/kg),and SSC low-and high-dose groups(2.5,10 mg/kg)using the random number table method,with 8 mice in each group.They were given water/relevant drugs,once a day,for 7 consecutive days.One hour after the last administration,all mice were intraperitoneally injected with 0.2％CCl4 olive oil to induce ALI model,except for the blank group.After 17 hours of the modeling,the liver index of mice was calculated.The levels of aspartate aminotransferase(AST),alanine aminotransferase(ALT),lactate dehydrogenase(LDH),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),and IL-1β in serum of mice were detected.The histopathological changes of liver tissue were observed.Meanwhile,the serum metabolomics of mice were analyzed by liquid chromatography-mass spectrometry.RESULTS Compared with the blank group,the levels of liver index,ALT,AST,LDH,TNF-α,IL-6,and IL-1β in the model group were significantly increased(P＜0.01).Hepatocytes were edema,vacuolar degeneration,more necrosis,and a large number of inflammatory cells were infiltrated.Compared with the model group,liver index and serum index levels of mice were significantly decreased(P＜0.05 or P＜0.01),accompanied by marked improvement in histopathological damage to the liver tissue.The metabolomics results showed that compared with the model group,there were 63 up-regulated and 256 down-regulated differential metabolites in the serum of mice in the SSC high-dose group,including prostaglandin B2,20-hydroxy-leukotriene B4,5-hydroxy-L-tryptophan,7α-hydroxycholesterol,etc.;these metabolites were primarily involved in metabolic pathways such as arachidonic acid metabolism,5-hydroxytryptamine synapse,primary bile acid biosynthesis.CONCLUSIONS SSC exerts a protective effect against CCl4-induced ALI by down-regulating the level of key metabolites such as prostaglandin B2 and 20-hydroxy-leukotriene B4,and then ruducing metabolic pathways such as arachidonic acid metabolism,5-hydroxytryptamine synapse,and primary bile acid biosynthesis.关键词
柴胡皂苷C/急性肝损伤/代谢组学/差异代谢物/花生四烯酸代谢/5-羟色胺突触/初级胆汁酸生物合成Key words
saikosaponin C/acute liver injury/metabolomics/differential metabolite/arachidonic acid metabolism/5-hydroxy-tryptamine synapse/primary bile acid biosynthesis分类
医药卫生引用本文复制引用
李新存,彭东辉,王永福,施亚敏,吴梦娟,付智慧,王娟..基于代谢组学研究柴胡皂苷C对急性肝损伤小鼠的保护作用[J].中国药房,2025,36(5):552-557,6.基金项目
河南省自然科学基金项目(No.242300420397) （No.242300420397）
河南省医学科技攻关计划联合共建项目(No.LHGJ20240259) （No.LHGJ20240259）
