中国药房2025，Vol.36Issue(5)：574-578,5.DOI:10.6039/j.issn.1001-0408.2025.05.12
马钱苷对脓毒症大鼠炎症反应及肠道屏障损伤的影响
Effects of loganin on inflammatory response and intestinal barrier damage in septic rats
摘要
Abstract
OBJECTIVE To investigate the effects of loganin on inflammatory response and intestinal barrier damage in septic rats by regulating the Ras homolog gene family member A(RhoA)/Rho-associated coiled-coil forming protein kinase 1(ROCK1)signaling pathway.METHODS A sepsis rat model was established by cecal ligation and puncture,and randomly divided into sepsis group,loganin low-dose group(50 mg/kg loganin,gavage),loganin high-dose group(200 mg/kg loganin,gavage),positive control group(0.2 mg/kg atorvastatin,intraperitoneal injection),and loganin high-dose+lysophosphatidic acid(LPA)group(200 mg/kg loganin gavage and intraperitoneal injection of 10 mg/kg RohA activator LPA).An additional sham surgery group was established.Each group consisted of 10 rats,and medications were administered once every 6 hours for 4 times.After 24 hours of the last intervention,the levels of serum inflammatory factors interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),and IL-1βwere detected.The pathological changes of ileal tissue were observed and Chiu's intestinal mucosal injury score was also performed.The levels of intestinal function-lactate dehydrogenase(D-lactate),D-amino acid oxidase(DAO)and endotoxin,the percentages of zonula occludens-1 protein(ZO-1)and Occludin positive staining area,as well as protein expressions of RhoA,and ROCK1 were all detected.RESULTS Compared with the sepsis group,the percentages of ZO-1 and Occludin positive areas increased significantly in loganin low-dose and high-dose groups;while the levels of IL-6,TNF-α,IL-1β,DAO,D-lactate and endotoxin,Chiu's intestinal mucosal injury score as well as protein expressions of RhoA and ROCK1 decreased significantly(P＜0.05);the destruction of rat ileal tissue was alleviated,and tissue edema and inflammatory infiltration were significantly reduced;moreover,the improvement effect in loganin high-dose group was superior to that in loganin low-dose group(P＜0.05).Compared with loganin high-dose group,RhoA activator LPA reversed the trend of changes in the above indicators(P＜0.05).CONCLUSIONS Loganin can alleviate inflammatory response and intestinal barrier damage in septic rats,the mechanism of which may be associated with inhibiting RhoA/ROCK1 signaling pathway.关键词
马钱苷/脓毒症/RhoA/ROCK1信号通路/炎症反应/肠道屏障损伤Key words
loganin/sepsis/RhoA/ROCK1 signaling pathway/inflammatory response/intestinal barrier damage分类
药学引用本文复制引用
王灿,李岩涛,周正,王璐鹏,高媛媛,范少希..马钱苷对脓毒症大鼠炎症反应及肠道屏障损伤的影响[J].中国药房,2025,36(5):574-578,5.基金项目
河北省中医药类科研计划课题(No.2022065,No.2020103) （No.2022065,No.2020103）
