中国医学科学院学报2025，Vol.47Issue(1)：1-9,9.DOI:10.3881/j.issn.1000-503X.16097
小核核糖核蛋白D1在胃癌中的表达水平及对患者预后的影响
Expression Level of Small Nuclear Ribonucleoprotein D1 in Gastric Cancer and Its Effect on Prognosis
摘要
Abstract
Objective To investigate the expression of small nuclear ribonucleoprotein D1(SNRPD1)in the gastric cancer tissue and evaluate the predictive value of SNRPD1 expression level for the long-term progno-sis of gastric cancer patients and the possible functioning mechanism of SNRPD1.Methods The UALCAN and Gene Expression Profiling Interactive Analysis(GEPIA)were employed to analyze the expression level of SNRPD1 in pan-cancer and its relationship with the prognosis of gastric cancer.The clinical data of 109 patients who under-went radical surgery for gastric cancer from January 2014 to January 2017 in the First Affiliated Hospital of Beng-bu Medical University were retrospectively analyzed.Gastric cancer and paracancerous tissue samples were collect-ed,and the expression of SNRPD1 was detected by immunohistochemical staining.Lentiviral transfection was em-ployed to construct the BGC-823 gastric cancer cell models with stable high and low expression of SNRPD1,respectively.The CCK-8 assay and colony formation assay were employed to measure the proliferation of gastric cancer cells,and flow cytometry was used to analyze the cell cycle.Western blotting was employed to determine the expression levels of proteins in the signaling pathway.Results The data from UALCAN and GEPIA showed that SNRPD1 was highly expressed in the tissue of malignant tumors including gastric cancer(P<0.001).The expression level of SNRPD1 in the gastric cancer tissue was higher than that in the paracancerous tissue(P<0.001).Moreover,the expression level of SNRPD1 was positively correlated with the levels of carcinoembryonic antigen(P<0.001),carbohydrate antigen 19-9(P<0.001),G stage(P=0.042),T stage(P=0.002),and N stage(P=0.027)in the patients with gastric cancer.The high expression of SNRPD1 had a predictive value for the long-term prognosis of gastric cancer(P<0.001),and it was an independent risk factor for the death of gastric cancer patients(P=0.003).The results of gene ontology and kyoto encyclopedia of genes and Genomes enrichment analyses showed that SNRPD1 was involved in the regulation of the cell cycle.The results of CCK-8 and colony formation assays showed that up-regulation of SNRPD1 promoted the proliferation of gastric cancer cells(P<0.001,P<0.001).The up-regulation of SNRPD1 up-regulated the expression of cyclin-de-pendent kinase 6 and G1/S-specific cyclin-D1(P<0.001,P=0.002),whereas the interference in SNRPD1 led to opposite results(P=0.004,P<0.001).SNRPD1 accelerated the G1/S phase transition of gastric canc-er cells(P<0.001).The overexpression of SNRPD1 promoted the expression of phosphorylated phosphatidyli-nositol 3-kinase(PI3K)and phosphorylated protein kinase B(Akt)in gastric cancer cells(P=0.043,P<0.001),whereas disruption of SNRPD1 inhibited their expression(both P<0.001).Insulin-like growth factor 1,an agonist of the PI3K/Akt signaling pathway,promoted the proliferation of gastric cancer cells with SNRPD1 disturbed(P=0.002).Conclusion High expression of SNRPD1 in the gastric cancer tissues is associated with poor prognosis,and it may promote tumor cell proliferation and regulate the cell cycle by activating the PI3K/Akt signaling pathway.关键词
胃癌/远期预后/小核核糖核蛋白D1/细胞周期/磷脂酰肌醇3-激酶/蛋白激酶B信号通路Key words
gastric cancer/long-term prognosis/small nuclear ribonucleoprotein D1/cell cycle/phosphatidylinositol 3-kinase/protein kinase B signaling pathway分类
临床医学引用本文复制引用
殷丽霞,杨晶晶,牛民主,耿志军,李江艳,李静..小核核糖核蛋白D1在胃癌中的表达水平及对患者预后的影响[J].中国医学科学院学报,2025,47(1):1-9,9.基金项目
安徽省高校协同创新项目(GXXT-2020-020)、安徽省高校优秀青年基金(2022AH030138)和安徽省卫生健康科研项目(AHWJ2022b088) （GXXT-2020-020）
