中华中医药杂志2025，Vol.40Issue(2)：516-523,8.
枳壳甘草汤对椎间盘退变大鼠生物钟基因表达的影响
Effects of Zhiqiao Gancao Decoction on the expression of circadian clock gene in intervertebral disc degeneration rats
摘要
Abstract
Objective:To investigate the effects of Zhiqiao Gancao Decoction on intervertebral disc degeneration(IDD)and the expression of circadian clock-related genes in the nucleus pulnosus tissue of a rat model.Methods:Sixty SD rats were randomly divided into sham group,model group,celecoxib group and Zhiqiao Gancao Decoction low-dose,medium-dose and high-dose groups,with 10 rats in each group.IDD model was constructed by needle puncture of the rat tail intervertebral disc.After respective interventions,samples were taken from each group.X-ray scanning and pathological staining were performed to detect pathological changes in the rat tail intervertebral disc.Enzyme-linked immunosorbent assay was used to detect the levels of inflammatory factors interleukin(IL)-1β,IL-4,IL-6,tumor necrosis factor-alpha(TNF-α)in the intervertebral disc tissue.Quantitative Real-time PCR was used to detect the expression levels of clock-related genes mRNA in the nucleus pulposus tissue,and Western Blot was used to detect the expression levels of extracellular matrix and circadian clock-related proteins in the nucleus pulposus tissue.Results:Compared with the sham group,the model group showed a significant decrease in intervertebral disc height index(P＜0.01),significant pathological changes in the intervertebral disc,significant increase in the expression of IL-1β,IL-6,TNF-α,ADAMTS4,MMP3 proteins(P＜0.01),significant decrease in the expression of IL-4,Aggrecan,Collagen Ⅱproteins,and Bmal1,Clock,Rev-erbα,Rorα,Perl,Cryl mRNA and proteins(P＜0.01).Compared with the model group,the celecoxib group and the Zhiqiao Gancao Decoction medium-dose and high-dose groups showed a significant increase in intervertebral disc height index(P＜0.01),alleviation of intervertebral disc pathological changes,significant decrease in the expression of IL-1β,IL-6,TNF-α,ADAMTS4,MMP3 proteins(P＜0.01),significant increase in the expression of IL-4,Aggrecan,Collagen Ⅱ proteins(P＜0.01,P＜0.05).In the celecoxib group,the expresion of Clock,Rev-erbα,Rorα,Per1,Cry1 mRNA and proteins were significantly increased(P＜0.01,P＜0.05);in the Zhiqiao Gancao Decoction high-dose group,the expression of Bmal1,Clock,Rev-erbα,Rorα,Perl,Cryl mRNA and proteins were significantly increased(P＜0.01).Conclusion:Zhiqiao Gancao Decoction can inhibit the inflammation of nucleus pulposus tissueand the degradation of extracelular matrix,and delay IDD,the internal mechanism may be related to the regulation of circadian clock genes.关键词
腰痛/椎间盘退变/生物钟/炎症因子/细胞外基质/枳壳甘草汤Key words
Low back pain/Intervertebral disc degeneration(IDD)/Circadian clock/Inflammatory factors/Extracellular matrix/Zhiqiao Gancao Decoction引用本文复制引用
黄泽灵,徐波,陆斌杰,蔡学峰,裴帅,刘昱江,祝学思,沈晓峰,李宇卫..枳壳甘草汤对椎间盘退变大鼠生物钟基因表达的影响[J].中华中医药杂志,2025,40(2):516-523,8.基金项目
国家自然科学基金面上项目(No.82174399),苏州市科技发展计划项目(No.SKYD2023150),江苏省研究生培养创新工程研究生实践创新计划(No.SJCX24_1023),江苏省中医药管理局重点项目(No.ZD202230),苏州市中医药管理局临床重点项目(No.LCZX202320),中医骨伤科学重点实验室建设项目(No.JSDW202253,No.SZS2022019),江苏省医学重点学科/实验室建设单位(No.苏卫科教[2022]17号) （No.82174399）
