中国医院用药评价与分析2025，Vol.25Issue(2)：134-136,142,4.DOI:10.14009/j.issn.1672-2124.2025.02.002
不同保存条件对全血标本雷帕霉素血药浓度测定结果的影响研究
Effects of Different Storage Conditions on Determination of Rapamycin Concentration in Whole Blood Samples
摘要
Abstract
OBJECTIVE:To study the effects of different storage conditions on determination of rapamycin concentration in whole blood samples.METHODS:A total of 49 discarded specimens from patients in the hospital who had completed rapamycin concentration tests from Dec.2021 to Apr.2023 were collected.Each specimen was mixed and divided into three groups,one for each group,and stored at room temperature(from 20 to 25℃),refrigerated(from 2 to 8℃),and frozen(from-30 to-20℃)for 7 d respectively.The blood concentration of rapamycin under various storage conditions was determined by chemiluminescent microparticle immunoassay.RESULTS:Compared with the blood concentration measured on the day of sampling,there was no significant difference in the average blood concentration of rapamycin at room temperature,refrigerated and frozen for 7 d(P>0.05).Compared with the rapamycin plasma concentration measured on the day of sampling,the blood concentration of rapamycin decreased in 21 cases of the samples stored at room temperature for 7 d,and the decrease was greater than 15％in 3 cases,with a variation range of 15.70％to 24.10％.After 7 d of refrigeration,compared with the original value,the concentration decreased in 29 cases,among which 9 cases had a concentration change greater than 15％,with a variation range of 15.17％to 39.52％.After 7 d of freezing,compared with the original value,the concentration decreased in 27 cases,among which 4 cases had a concentration change greater than 15％,with a variation range of 15.98％to 21.59％.CONCLUSIONS:If the rapamycin sample cannot be detected in time on the day of sampling,it can be preserved at room temperature or frozen away from light,and the determination should be completed within 7 d.关键词
雷帕霉素/治疗药物监测/保存条件/化学发光微粒子免疫检测法Key words
Rapamycin/Therapeutic drug monitoring/Storage conditions/Chemiluminescence microparticle immunoassay分类
医药卫生引用本文复制引用
权丹妮,陈井霞,杨云云,王卓,张凌鹏,高丽红..不同保存条件对全血标本雷帕霉素血药浓度测定结果的影响研究[J].中国医院用药评价与分析,2025,25(2):134-136,142,4.基金项目
国家自然科学基金资助项目(No.82304619) （No.82304619）
