摘要
Abstract
Objective To analyze the mechanism of Shenqi Dihuang Decoction in the treatment of diabetic nephropathy(DN)based on network pharmacology and molecular docking.Methods The active components of Shenqi Dihuang Decoction were retrieved using TCMSP,the active components related targets were obtained from the PubChem and SwissTargetPrediction databases,and protein targets were supplemented and standardized through UniProt database.DN related targets were obtained through DisGeNET and GeneCards databases.Intersecting targets were obtained using Venny 2.1.0 platform.Simultaneously importing STRING database for analysis and constructing protein-protein interaction(PPI)network using Cytoscape 3.10.0 software,GO and KEGG pathway enrichment analysis were performed using Metascape database,and the analysis results were visualized using the Microbiotics platform.Molecular docking was performed using AutoDockTools 1.5.7 software and molecular docking pattern diagrams were drawn using PyMOL 2.6.0 software.Results A total of 78 active components were retrieved from Shenqi Dihuang Decoction,with the main active components including kaempferol,quercetin and hederagenin.Among them,there were 164 core targets related to DN,including IL6,TNF and AKT1.The GO function enrichment results mainly involved cell response to hormone,membrane raft,kinase activity,etc.;KEGG pathway enrichment analysis mainly involved cancer pathways,PI3K-Akt signaling pathways and lipids and atherosclerosis.The molecule docking results showed that the core active components and the core targets docking activity was strong.Conclusion Shenqi Dihuang Decoction can exert therapeutic effects on DN through core targets such as IL6,TNF,AKT1 and activation of cancer pathways,PI3K-Akt signaling pathways and lipids and atherosclerosis.关键词
参芪地黄汤/糖尿病肾病/网络药理学/分子对接Key words
Shenqi Dihuang Decoction/diabetic nephropathy/network pharmacology/molecular docking分类
医药卫生
