中国中药杂志2025，Vol.50Issue(4)：896-907,12.DOI:10.19540/j.cnki.cjcmm.20241014.703
基于肠道菌群及代谢组学探讨白芷多糖对溃疡性结肠炎小鼠的治疗及其作用机制
Angelicae Dahuricae Radix polysaccharides treat ulcerative colitis in mice by regulating gut microbiota and metabolism
摘要
Abstract
This study employed 16S rRNA gene high-throughput sequencing and metabolomics to explore the mechanism of Angelicae Dahuricae Radix polysaccharides(RP)in the treatment of ulcerative colitis(UC).A mouse model of UC was induced with 2.5%dextran sulfate sodium.The therapeutic effects of RP on UC in mice were evaluated based on changes in body weight,disease activity index(DAI),and colon length,as well as pathological changes.RT-qPCR was performed to assess the mRNA levels of interleukin(IL)-6,IL-1β,tumor necrosis factor(TNF)-α,myeloperoxidase(MPO),mucin 2(Muc2),Occludin,Claudin2,and ZO-1 in the mouse colon tissue.ELISA was employed to measure the expression of IL-1β and TNF-α in the colon tissue.The intestinal permeability of mice was evaluated by the fluorescent dye permeability assay.Immunohistochemistry was employed to detect the expression of Muc2 and occludin in the colon tissue.Changes in gut microbiota and metabolites were analyzed by 16S rRNA sequencing and ultra-high-performance liquid chromatography coupled with quadrupole-orbitrap mass spectrometry(UPLC-Q-Exactive Plus Orbitrap MS),respectively.The results indicated that low-dose RP alleviated general symptoms,reduced colonic inflammation and intestinal permeability,and promoted Muc2 secretion and tight junction protein expression in UC mice.In addition,low-dose RP increased gut microbiota diversity in UC mice and decreased the relative abundance of harmful bacteria such as Ochrobactrum and Streptococcus.Twenty-seven differential metabolites were identified in feces,and low-dose RP restored the levels of disturbed metabolites.Notably,arginine and proline metabolism were the most significantly altered amino acid metabolic pathways following low-dose RP intervention.In conclusion,RP can ameliorate general symptoms,inhibit colonic inflammation,and maintain intestinal mucosal barrier integrity in UC mice by modulating gut microbiota composition and arginine and proline metabolism.关键词
白芷多糖/溃疡性结肠炎/肠道菌群/代谢组学Key words
Angelicae Dahuricae Radix polysaccharides/ulcerative colitis/gut microbiota/metabolomics引用本文复制引用
徐锋,朱磊,李亚楠,程成,崔媛,童依姮,胡静怡,沈洪..基于肠道菌群及代谢组学探讨白芷多糖对溃疡性结肠炎小鼠的治疗及其作用机制[J].中国中药杂志,2025,50(4):896-907,12.基金项目
国家中医药管理局高水平中医药重点学科项目(中医脾胃病学)(国中医药人教函[2023]85号) （中医脾胃病学）
全国名老中医药专家传承工作室项目(国中医药人教函[2022]75号) （国中医药人教函[2022]75号）
国家自然科学基金面上项目(82274483) （82274483）
江苏省中医药管理局重点项目(ZD202212) （ZD202212）
江苏省研究生科研与实践创新计划项目(KYCX22_1938) （KYCX22_1938）
