中国中药杂志2025，Vol.50Issue(4)：959-973,15.DOI:10.19540/j.cnki.cjcmm.20241218.201
基于血浆药物化学结合网络药理学及药代动力学探究咳宁颗粒止咳祛痰的质量标志物
Prediction of quality markers for cough-relieving and phlegm-expelling effects of Kening Granules based on plasma pharmacology combined with network pharmacology and pharmacokinetics
摘要
Abstract
This study predicts the quality markers(Q-markers)for the cough-relieving and phlegm-expelling effects of Kening Gran-ules based on pharmacodynamics,plasma drug chemistry,network pharmacology,and pharmacokinetics.Strong ammonia solution spray and phenol red secretion assays were employed to evaluate the cough-relieving and phlegm-expelling effects of Kening Granules.Twenty-six absorbed prototype components of Kening Granules were identified by ultra high performance liquid chromatography coupled with Q-Exactive Plus quadrupole/Orbitrap high resolution mass spectrometry(UHPLC-Q-Exactive Plus Orbitrap HRMS).Through network phar-macology,11 potential active components were screened out for the cough-relieving and phlegm-expelling effects of Kening Granules.The 11 components acted on 40 common targets such as IL6,TLR4,and STAT3,which mainly participated in PI3K/Akt,HIF-1,and EGFR signaling pathways.Pharmacokinetic quantitative analysis was performed for 7 prototype components.Three compounds including azelaic acid,caffeic acid,and vanillin were identified as Q-markers for the cough-relieving and phlegm-expelling effects of Kening Granules based on their effectiveness,transmissibility,and measurability.The results of this study are of great significance for clarifying the phar-macological substance basis,optimizing the quality standards,and promoting the clinical application of Kening Granules.关键词
咳宁颗粒/止咳祛痰/网络药理学/质量标志物Key words
Kening Granules/cough-relieving and phlegm-expelling effects/network pharmacology/quality marker引用本文复制引用
陈轻轻,张元现,王倩,张金玲,郑林,黄勇,金阳,巩仔鹏,李月婷..基于血浆药物化学结合网络药理学及药代动力学探究咳宁颗粒止咳祛痰的质量标志物[J].中国中药杂志,2025,50(4):959-973,15.基金项目
贵州省科技支撑项目(黔科合支撑[2022]一般188) （黔科合支撑[2022]一般188）
贵州省科技计划项目(黔科合平台人才-CXTD[2023]019) （黔科合平台人才-CXTD[2023]019）
贵州省高层次创新型人才项目(黔科合平台人才-GCC[2023]036,黔科合平台人才-GCC[2022]031-1) （黔科合平台人才-GCC[2023]036,黔科合平台人才-GCC[2022]031-1）
