浙江大学学报（医学版）2025，Vol.54Issue(1)：49-57,9.DOI:10.3724/zdxbyxb-2024-0218
绞股蓝乙醇提取物通过抑制细胞凋亡改善小鼠帕金森样行为
Gynostemma pentaphyllum ethanol extract ameliorates motor dysfunction in a Parkinson's disease mouse model through inhibiting neuronal apoptosis
摘要
Abstract
Objective:To investigate the protective effects and underlying mechanisms of Gynostemma pentaphyllum(GP)ethanol extract on motor dysfunction in a mouse model of Parkinson's disease(PD).Methods:Eighty C57BL/6 male mice were randomly divided into five groups:control group,model group,levodopa group(positive control group),low-dose GP group,and high-dose GP group,with 16 mice per group.The PD model was induced by injection of 6-hydroxydopamine into the substantia nigra pars reticulata of the mice.Two weeks after 6-hydroxydopamine,positive control group received intraperitoneal injection of levodopa 10 mg·kg-1·d-1,while low-dose GP and high-dose GP groups received GP extract 100 or 200 mg·kg-1·d-1 orally for three weeks.After a 3-week-treatment,the effects of GP on motor dysfunction in 6-hydroxydopamine-induced PD were assessed using open field and CatWalk gait tests,while the effects on muscle strength were evaluated by forelimb grip strength.Immunofluorescence staining was used to detect the number of tyrosine hydroxylase(TH)positive neurons.The levels of dopamine and serotonin in the midbrain were determined by enzyme-linked immunosorbent assay.In addition,Western blotting was performed to detect the expression of mitogen-activated protein kinase(MAPK)family proteins such as p-extracellular signal-regulated kinase(ERK)1/2,p-p38 and p-c-Jun N-terminal kinase(JNK)1/2,and mitochondrial apoptosis pathway proteins such as B-cell lymphoma(Bcl)-2,Bcl-2 associated X protein(Bax),and cleaved-cysteine aspartic acid specific protease(caspase)-3.Results:Behavioral experiments showed that GP significantly improved the spontaneous activity and motor coordination of PD mice(P<0.05).The forelimb grip strength was also increased by GP treatment(P<0.05),compared to the PD model group.In addition,compared with the model group,the number of TH-positive neurons in substantia nigra pars reticulata region,the levels of dopamine and serotonin in midbrain and the expression of p-ERK1/2 were significantly increased by GP treatment(all P<0.05),whereas the expression of p-p38 and p-JNK1/2,the ratio of Bax/Bcl-2 and cleaved-caspase-3/caspase-3 were significantly decreased(all family proteins and the expression of mitochondrial apoptosis-related proteins,thereby ameliorating motor deficits in PD mice.关键词
帕金森病/绞股蓝/行为学实验/多巴胺能神经元/丝裂原活化蛋白激酶家族蛋白/线粒体凋亡相关蛋白/小鼠Key words
Parkinson's disease/Gynostemma pentaphyllum/Behavioral experiment/Dopaminergic neuron/Mitogen-activated protein kinase family proteins/Mitoptosis-related proteins/Mice分类
医药卫生引用本文复制引用
赵婷婷,何岚峤,严森,樊鹏雨,张翀,曾玲晖..绞股蓝乙醇提取物通过抑制细胞凋亡改善小鼠帕金森样行为[J].浙江大学学报（医学版）,2025,54(1):49-57,9.基金项目
浙江省自然科学基金(LTGD23C090001) （LTGD23C090001）
国家自然科学基金(31800888) （31800888）
浙大城市学院科研培育基金(X-202104,X-202304) This work was supported by the Natural Science Foundation of Zhejiang Province(LTGD23C090001),National Natural Science Foundation of China(31800888),and Scientific Research Foundation of Hangzhou City University(X-202104,X-202304) （X-202104,X-202304）
