中南民族大学学报（自然科学版）2025，Vol.44Issue(3)：357-364,8.DOI:10.20056/j.cnki.ZNMDZK.20240727
Biphalin激活PI3K/Akt通路减轻氧糖剥夺/复氧诱导的神经元损伤
Biphalin alleviated oxygen-glucose deprivation/reoxygenation-induced neuronal injury by activating PI3K/Akt signaling pathway
摘要
Abstract
To investigate the effects and mechanism of biphalin on primary cortical neuronal injury induced by oxygen-glucose deprivation/reoxygenation(OGD/R),neurons were randomly divided into five groups,namely the control group(without intervention),the OGD/R group(underwent OGD/R only),the OGD/R+biphalin group(treated with 0.01,0.1,1,and 10 nmol/L biphalin during OGD/R),the OGD/R+biphalin+naloxone group(treated with 0.1 nmol/L biphalin and 0.1 mmol/L naloxone during OGD/R),the OGD/R+biphalin+LY294002 group(treated with 0.1 nmol/L biphalin and 20 μmol/L LY294002 during OGD/R).Cell counting kit-8(CCK-8),lactate dehydrogenase(LDH)assays and propidium iodide(PI)staining were performed to analyse the effects of biphalin on primary cortical neurons.The protein expression levels of Bcl-2,Bax,cleaved-caspase-3,p-PI3K,PI3K,p-Akt and Akt were measured by Western blotting.Due to the greatest neuroprotection of biphalin was observed at 0.1 nmol/L,this concentration was chosen for subsequent studies.The decreased cell viability and increased cell death and LDH release were observed after OGD/R compared to the control group.The levels of cleaved-caspase-3 and Bax significantly increased in OGD/R group compared with the control group.Compared with the OGD/R group,biphalin significantly increased the survival rate and the level of Bcl-2 and the ratios of p-PI3K/PI3K and p-Akt/Akt,reduced LDH release and the protein levels of cleaved-caspase-3 and Bax.However,both of them were mostly suppressed by the opioid antagonist naloxone and the PI3K inhibitor LY294002 respectively.These results suggest that biphalin protected against OGD/R injury in primary cortical neurons by activating PI3K/Akt signaling pathway via opioid receptor.关键词
biphalin肽/新生儿缺氧缺血性脑损伤/氧糖剥夺/复氧(OGD/R)/PI3K/Akt通路/原代皮层神经元细胞Key words
biphalin/neonatal hypoxic-ischemic brain injury/oxygen-glucose deprivation/reoxygenation/PI3K/Akt signaling pathway/primary cortical neurons分类
药学引用本文复制引用
陈玲,张锦佳,黄宇钧,陆媛,闵加威..Biphalin激活PI3K/Akt通路减轻氧糖剥夺/复氧诱导的神经元损伤[J].中南民族大学学报（自然科学版）,2025,44(3):357-364,8.基金项目
国家自然科学基金资助项目(82301574) （82301574）
湖北省自然科学基金资助项目(2024AFB875) （2024AFB875）
中央高校基本科研业务费专项资金资助项目(CZQ23030) （CZQ23030）
中南民族大学科学研究基金资助项目(YZZ19018) （YZZ19018）
