植物学报2025，Vol.60Issue(1)：17-32,16.DOI:10.11983/CBB24038
水稻OsWAK16通过调节抗氧化酶活性调控种子抗老化能力
OsWAK16 Regulates Seed Anti-aging Ability by Modulating Antioxidant Enzyme Activity in Rice
摘要
Abstract
INTRODUCTION:The cell wall-associated kinase(WAK)family has annotated approximately 130 WAK genes in the genome of rice(Oryza sativa),which play an important role in rice growth and development and stress responses. RATIONALE:Here,we investigated the regulation and physiological mechanism of OsWAK16,an encoding gene of the cell wall-associated kinase WAK16-RLK,on rice seed vigor and anti-aging ability. RESULTS:The results showed that before and after artificial aging,the seed vigor of OsWAK16 knock out mutants and overexpression lines was significantly lower and higher than that of wild-type seeds,respectively,indicating that Os-WAK16 positively regulates the anti-aging ability of seeds.Physiological and biochemical analyses indicated that com-pared with wild-type seeds before and after artificial aging treatment,malondialdehyde(MDA)content and electrical conductivity(EC)of seed soaking solution of OsWAK16 knock out mutant seeds were significantly increased,while anti-oxidant enzyme activity was significantly decreased.The reverse was true in overexpression seeds.In addition,the dif-ferential expression of OsWAK16 in three types of seeds,whether artificially aged or not,also caused synergistic changes in the expression of other seed vigor-related genes OsPER1A,OsbZIP23,OsPIMT1,OsSdr4,OsMSRB5 and OsHSP18.2. CONCLUSION:Therefore,it is speculated that OsWAK16 may work synergistically with other seed vigor-related genes to clear reactive oxygen species in cells,thereby regulating seed vigor and anti-aging capacity.关键词
OsWAK16/种子活力/人工老化/丙二醛/抗氧化酶Key words
OsWAK16/seed vigor/artificial aging/malondialdehyde/antioxidant enzyme引用本文复制引用
田建红,刘燕,尹梦琪,王静,陈婷,汪燕,姜孝成..水稻OsWAK16通过调节抗氧化酶活性调控种子抗老化能力[J].植物学报,2025,60(1):17-32,16.基金项目
国家自然科学基金(No.32072125)和湖南省发育生物学工程与新产品协同创新中心(No.20134486) （No.32072125）
