植物学报2025，Vol.60Issue(1)：49-61,13.DOI:10.11983/CBB24019
甘蓝型油菜转录因子BnaABF2的表征分析及互作蛋白鉴定
Analysis of Expression Characteristics and Identification of Interaction Proteins of BnaABF2 Transcription Factor in Brassica napus
摘要
Abstract
ABF transcription factors are collectively referred to as basic leucine zipper proteins that can specifically re-cognize and bind to ABA-responsive elements(ABRE),participating in ABA signal transduction and serving as regulators of ABA signal transcriptional responses.This study analyzed the protein encoded by the BnaABF2 gene in Brassica napus.Subcellular localization results showed that the BnaABF2 protein is localized in the nucleus.Analysis of transcrip-tional activity in the yeast system indicated that BnaABF2 has no transcriptional activation activity;qRT-PCR detection re-vealed that the expression level of BnaABF2 is highest in leaves.We also found that ABA treatment,simulated drought,and salt stress can induce the expression of BnaABF2;BiFC results showed that BnaMPK1/2/6/7/9/12/13 can interact with BnaABF2.Dual-LUC results suggested that BnaMPK7 may enhance the transcriptional regulation of BnaABF2 on down-stream target genes through phosphorylation.This study initially explored the basic characteristics and interacting proteins of the transcription factor BnaABF2,providing theoretical guidance for understanding its functions and mechanisms.关键词
甘蓝型油菜/ABF2/亚细胞定位/MPK/互作蛋白Key words
Brassica napus/ABF2/subcellular localization/MPK/interaction proteins引用本文复制引用
杨柳卿,王劲,燕敬利,陈芹芹,程浩坤,李春,赵培玉,杨博,江元清..甘蓝型油菜转录因子BnaABF2的表征分析及互作蛋白鉴定[J].植物学报,2025,60(1):49-61,13.基金项目
国家自然科学基金(No.32171953) （No.32171953）
