植物学报2025，Vol.60Issue(1)：62-73,12.DOI:10.11983/CBB24068
甘蓝型油菜BnaA02.CPSF6基因的克隆及功能分析
Cloning and Functional Analysis of the BnaA02.CPSF6 Gene from Brassica napus
摘要
Abstract
INTRODUCTION:The CPSF family(cleavage and polyadenylation specificity factor)is a crucial protein family that is responsible for polyadenylation signal recognition in mRNA precursors,cleavage and the addition of poly(A)tails to mRNAs in plants.This family plays crucial roles in the regulation of flowering time,the environmental response,and seed development.Currently,the function of the CPSF family genes in Brassica napus is unclear. RATIONALE:To explore the function and expression patterns of the CPSF gene family,this study cloned BnaA02.CPSF6 from B.napus variety Zhongshuang No.11 and conducted bioinformatics analysis,subcellular localization,expression pattern,and functional characterization of the gene. RESULTS:These results indicate that the coding region of the BnaA02.CPSF6 gene is 1 938 bp in length and encodes 646 amino acids without intron structures.Its promoter region contains multiple cis-acting elements involved in light re-sponses and MYB binding sites.Additionally,there are six genes homologous to BnaA02.CPSF6 in B.napus.The BnaA02.CPSF6 gene expressed in the roots,stems,leaves,flowers and different developmental seeds of B.napus,es-pecially significantly higher in 15-35 d developmental seeds,and its encoded protein was localized in the nucleus.The BnaA02.CPSF6 gene expression is upregulated under salt and drought stress.Under treatment with hormones such as ABA,IAA,GA3,SA,and MeJA,the expression of BnaA02.CPSF6 gene is initially inhibited and then gradually recovers to normal levels.Under normal conditions,the overexpression of the BnaA02.CPSF6 gene in Arabidopsis thaliana results in an early bolting phenotype,along with a reduced number of rosette leaves. CONCLUSION:In summary,the above results indicate that the BnaA02.CPSF6 is involved in abiotic stress responses,is regulated by phytohormones,and may also play a promoting role in flowering regulation.关键词
甘蓝型油菜/BnaA02.CPSF6/基因功能/表达模式/生长发育Key words
Brassica napus/BnaA02.CPSF6/gene function/expression patterns/growth and development引用本文复制引用
李青洋,刘翠,何李,彭姗,马嘉吟,胡子祎,刘宏波..甘蓝型油菜BnaA02.CPSF6基因的克隆及功能分析[J].植物学报,2025,60(1):62-73,12.基金项目
国家自然科学基金(No.32071929)、浙江省自然科学基金(No.LY21C130001)、浙江省"十四五"农业新品种选育重大科技专项子课题(No.2021C02064-2-1)和浙江农林大学科研发展基金(No.2021FR044) （No.32071929）
