植物学报2025，Vol.60Issue(1)：81-89,9.DOI:10.11983/CBB24022
成熟禾谷类作物种子胚乳细胞的显微CT扫描方法
A New Cereal Seed Treatment Method for Displaying Endosperm Cell Structures Under Micro CT Scanning
摘要
Abstract
Cereal starch endosperm is the main source of human staple food,but the methods for observing its mature cell structure are not well developed.Micro CT technology,a non-destructive three-dimensional imaging technique,is a powerful tool for studying plant morphology.However,due to the uniform density of cereal starch endosperm,whose cell structures cannot be distinguished clearly by conventional micro CT techniques.In this study,we used phosphotungstic acid to treat ten different kinds of cereal seeds of seven crops,and then dried them by CO2 critical point dryer.We found that the cell structure of the treated endosperms can be clearly displayed by micro CT.Our method provides a new way for studying the structures and functions of cereal starch endosperm cells.关键词
显微CT/禾谷类淀粉胚乳/磷钨酸Key words
micro CT/cereal starch endosperm/phosphotungstic acid引用本文复制引用
徐秀苹,杨小雨,冯旻..成熟禾谷类作物种子胚乳细胞的显微CT扫描方法[J].植物学报,2025,60(1):81-89,9.基金项目
中国科学院技术支撑人才项目(2022)、黔农科院青年科技基金(No.2021-20)和黔农科院国基后补助(No.2021-12) （2022）
