植物学报2025，Vol.60Issue(1)：101-113,13.DOI:10.11983/CBB24048
单细胞转录组学在植物生长发育及胁迫响应中的应用进展
Advances in the Application of Single-cell Transcriptomics in Plant Growth,Development and Stress Response
摘要
Abstract
Single-cell transcriptomics has improved the spatiotemporal resolution from multi-cell to single-cell levels,and notable progress in this technique has facilitated the identification of new rare cell types,exploration of intercellular hete-rogeneity,and mapping of cell developmental trajectories.Single-cell transcriptomics is currently being widely used in various research fields such as plant growth and development,stress response,and environmental adaptability,which helps to more thoroughly and precisely uncover the molecular regulatory mechanisms underlying plant life processes.However,there are numerous challenges associated with the study and analysis of different plant species.In this review,we compare and evaluate various single-cell transcription techniques and processes,summarize plant single-cell studies in recent years,and explore new single-cell analysis tools to support researchers studying plant biology with high preci-sion and dynamics.In addition,we propose future directions in using single-cell transcriptomics technologies to address some of the key challenges in plant research and breeding.Furthermore,some important methods for addressing plant research and breeding with single-cell transcriptomics are discussed,along with their difficulties and potential applica-tions.关键词
植物生长发育/胁迫响应/单细胞转录组测序/10× Genomics/细胞类型Key words
plant growth and development/stress response/single-cell RNA sequencing(scRNA-seq)/10× Genomics/cell type引用本文复制引用
王亚萍,包文泉,白玉娥..单细胞转录组学在植物生长发育及胁迫响应中的应用进展[J].植物学报,2025,60(1):101-113,13.基金项目
国家自然科学基金(No.32360402)和内蒙古自治区高等学校科学研究项目(No.B20231093Z) （No.32360402）
