植物遗传资源学报2025，Vol.26Issue(3)：470-480,11.DOI:10.13430/j.cnki.jpgr.20240602001
水稻核心种质耐盐鉴定与分子标记开发应用
Identification of Salt Tolerance in Rice Core Germplasm and Development and Application of Molecular Markers
摘要
Abstract
Rice is a salt-sensitive plant,and soil salinization significantly impacts rice performance.Therefore,exploring excellent haplotypes of salt-tolerant genes and generating elite germplasm are of great significance for rice breeding.In this study,we firstly analyzed 236 core germplasm lines collected from the 3K Rice Genome Project Database,for salt tolerance during the seedling stage and throughout the entire growth period in the field.A highly salt-tolerant germplasm'71011'from Australia was identified.This germplasm survived for 25.5 d and exhibited a salt tolerance level of 5.2 under 150 mmol/LNaC1 treatment conditions,with a 100％survival rate under 0.3％to 0.5％salt treatment in the paddy field.We further conducted haplotype analysis on 20 reported salt tolerance genes in the core collection.Eight genes,including AKT1,CPK12,MYB48,P5CS1,SIK1,SKC1,SNAC1,and HKT1,were identified as having haplotypes associated with salt tolerance.One salt-tolerant variety'Yanfeng 47'and one normal variety'Nongken 57'were selected as parents to generate recombinant inbred lines.Finally,three molecular markers,which have been confirmed by PCR and qRT-PCR,were designed for selecting elite haplotypes of three genes,AKT1,MYB48,and HKT1.Through marker-assisted selection,we successfully developed three high salt-tolerance lines by aggregating these elite haplotypes.Our results provide available germplasm resources,molecular markers and innovative lines for breeding new salt-tolerant rice varieties.关键词
水稻种质资源/耐盐鉴定/单倍型分析/分子标记Key words
rice germplasm resources/salt tolerance identification/haplotype analysis/molecular markers引用本文复制引用
王世壮,陈子易,郑晓明,王文生,杨庆文,乔卫华,聂亚敏,黄婧芬,张巧玲,郑崇珂,谢先芝,王艳艳,邢梦,陈文熹..水稻核心种质耐盐鉴定与分子标记开发应用[J].植物遗传资源学报,2025,26(3):470-480,11.基金项目
国家重点研发项目(2021YFD1200100) （2021YFD1200100）
中国农业科学院南繁专项(YYLH2402)National Key R&D Program of China(2021YFD1200100) （YYLH2402）
Nanfan Special Project of Chinese Academy of Agricultural Sciences(YYLH2402) （YYLH2402）
