中外医学研究2025，Vol.23Issue(6)：4-7,4.DOI:10.14033/j.cnki.cfmr.2025.06.002
转化生长因子β1诱导的调节性T细胞抑制炎性骨缺损吸收的修复机制研究
Study on the Repair Mechanism of Regulatory T Cells Transforming Growth Factor β1 Induced by to Inhibit the Absorption of flammatory Bone Defect
摘要
Abstract
Objective:To analyze the repair mechanism of Transforming Growth Factor-betal(TGF-β1)induced by regulatory T cells to inhibiting the absorption of inflammatory bone defect.Method:A total of 25 healthy New Zealand white rabbits were selected to construct a high-specificity animal model of femoral condylar bone defect of knee joint.Finally,20 animal models were successfully constructed and randomly divided into group A and group B with 10 rabbits each,and 10 healthy New Zealand white rabbits were included as a blank control group.The group A were injected subcutaneously with 5 ng/mL TGF-β1 solution(2 days/time)on both knee joints,the blank control group were injected with equal volume of normal saline,group B were not injected,and rabbits in the three groups were routinely fed for 14 days.The levels of serum inflammatory factors and the expressions of microbial DNA methyltransferase 1(DNMT1),TGF-β1,RUNt-related transcription factor 2(Runx2)and osteopontin(OPN)in femoral condylar bone defect of knee joint of rabbits were compared among the three groups.Result:The serum levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and C-reactive protein(CRP)in group B were higher than those in group A and blank control group,and the differences were statistically significant(P<0.05).There was no significant difference in serum TNF-α,IL-6 and CRP levels between group A and blank control group(P>0.05).The expressions of DNMT1,TGF-β1,Runx2 and OPN in femoral condylar bone defect tissue of knee joint of rabbits in group B were lower than those in group A and blank control group,and the differences were statistically significant(P<0.05).Conclusion:TGF-β1 can alleviate the inflammatory response of high specificity rabbit knee joint femoral condylar bone defects and promote osteogenic differentiation.关键词
炎性骨缺损/转化生长因子β1/调节性T细胞/修复Key words
Inflammatory bone defects/Transforming growth factor β1/Regulatory T cells/Repair引用本文复制引用
杨俊龙,梁惠全,欧泽锋,林锦浩,白荣飞,张梓杰,王国亮..转化生长因子β1诱导的调节性T细胞抑制炎性骨缺损吸收的修复机制研究[J].中外医学研究,2025,23(6):4-7,4.基金项目
广东省医学科学技术研究基金项目(A2023234) （A2023234）
广东省中医药局科研项目(20231250) （20231250）
