中外医学研究2025，Vol.23Issue(6)：142-146,5.DOI:10.14033/j.cnki.cfmr.2025.06.038
miR-212-3p靶向MeCP2调节BDNF/TrkB通路对神经病理性疼痛的影响
The Effect of miR-212-3p Targeting MeCP2 on the Regulation of BDNF/TrkB Pathway on Neuropathic Pain
摘要
Abstract
Objective:To investigate the role of miR-212-3p-mediated epigenetic modification of BDNF/TrkB targeting MeCP2 in neuropathic pain in vitro and in vivo.Method:SD rats were divided into 3 groups:blank control group(Black group),Sham group(Sham group)and chronic constriction injury group(CCI group).The paw withdrawal mechanical threshold(PWMT)and paw withdrawal thermal latency(PWTL)of rats were monitored one day after surgery,and the mRNA expressions of miR-212-3p,MeCP2,brain-derived neurotrophic factor(BDNF)and TrkB in spinal cord tissues were detected by RT-PCR.The content of MeCP2,BDNF and TrkB proteins in rat spinal cord were detected by Western Blot.In vitro miR-212-3p overexpression plasmid was transfected into spinal cord neuronal DRG cells,and the mRNA and protein expressions of MeCP2,BDNF and TrkB were detected by RT-PCR and Western Blot,respectively Result:In CCI group,there were some behavioral abnormalities,such as flapping feet,licking feet and drawing toes together.Compared with Sham group,PMWT and PWTL values were decreased,and the difference was statistically significant(P<0.05).The expression of miR-212-3p was up-regulated,the mRNA and protein expressions of MeCP2 were down-regulated,while the mRNA and protein expressions of BDNF and TrkB were up-regulated,with statistical significance(P<0.05).Compared with the Black group,the expression of MeCP2 mRNA and protein of miR-212-3p mimic cells decreased,while the expression of BDNF and TrkB mRNA and protein were significantly up-regulated(P<0.05).Compared with Black group,the above index results of miR-212-3p inhibitor group were opposite(P<0.05).Conclusion:Inhibition of miR-212-3p can up-regulate MeCP2,thereby down-regulating BDNF/TrkB pathway through MeCP2 epigenetic modification and relieving neuropathic pain.关键词
MeCP2/神经病理性疼痛/BDNF/TrkB/miR-212-3p/表观遗传学Key words
MeCP2/Neuropathic pain/BDNF/TrkB/miR-212-3p/Epigenetics引用本文复制引用
赵辉,梁雪,王玲,杨少杰,丰晖..miR-212-3p靶向MeCP2调节BDNF/TrkB通路对神经病理性疼痛的影响[J].中外医学研究,2025,23(6):142-146,5.基金项目
甘肃省自然科学基金项目(22JR5RA737) （22JR5RA737）
