中药药理与临床2025，Vol.41Issue(1)：28-32,5.
六味地黄汤下调NF-κB/NLRP3信号通路抑制HK-2细胞焦亡延缓肾纤维化
Liuwei Dihuang Decoction(六味地黄汤)Inhibits Pyroptosis of HK-2 Cells and Delays Renal Fibrosis by Downregulating NF-κB/NLRP3 Signaling Pathway
摘要
Abstract
Objective:To investigate the therapeutic effect and mechanism of Liuwei Dihuang Decoction(六味地黄汤)on renal fibrosis in a model of transforming growth factor β1(TGF-β1)-induced renal tubular epithelial cells(HK-2).Methods:HK-2 cells were cultured in vitro and ran-domly divided into the following groups:normal control group,model control group,Liuwei Dihuang Decoction-medicated serum 5%,10%,and 20%groups,and MCC950 1 μmol/L group.Except for the normal control group,the other groups were induced with 10 ng/mL TGF-β1 to in-duce a renal fibrosis model and were intervened with corresponding drugs.After 24 hours of administration,cells were collected and the expres-sion of NF-κB/NLRP3 signaling pathway and related indicators of pyroptosis and renal fibrosis were measured using RT-qPCR,Western blot,and immunofluorescence methods.Results:Compared with the normal control group,the model control group had upregulated expression of NF-κB,Caspase-1,IL-1β,IL-18,and Vimentin proteins in HK-2 cells(P<0.05),downregulated expression of E-cadherin protein(P<0.05),and upreg-ulated mRNA expression levels of Nf-κb,Nlrp3,Caspase-1,and Il-18(P<0.05).Compared with the model control group,the Liuwei Dihuang Decoction-medicated serum 10%and 20%groups and the MCC950 1 μmol/L group had downregulated expression of NF-κB,Caspase-1,IL-1β,IL-18,and Vimentin proteins in HK-2 cells(P<0.05),upregulated expression of E-cadherin protein(P<0.05),and downregulated mRNA ex-pression levels of Nf-κb,Nlrp3,Caspase-1,and Il-18(P<0.05).Conclusion:Liuwei Dihuang Decoction can inhibit pyroptosis in renal tubular epithelial cells and delay renal fibrosis,potentially through the downregulation of the NF-κB/NLRP3 signaling pathway.关键词
六味地黄汤/细胞焦亡/肾纤维化/HK-2细胞/核因子κB/核苷酸结合寡聚化结构域样受体蛋白3信号通路Key words
Liuwei Dihuang Decoction(六味地黄汤)/Pyroptosis/Renal Fibrosis/HK-2 Cell/NF-κB/NLRP3 Signaling Pathway引用本文复制引用
马秀英,黄娜,杨成,汪涵俪,裴刚,孟斌,唐群..六味地黄汤下调NF-κB/NLRP3信号通路抑制HK-2细胞焦亡延缓肾纤维化[J].中药药理与临床,2025,41(1):28-32,5.基金项目
湖南省自然科学基金项目(编号:2021JJ30506) （编号:2021JJ30506）
湖南省教育厅重点项目(编号:21A0242) （编号:21A0242）
湖南中医药大学科研项目(编号:Z2023XJYB04) （编号:Z2023XJYB04）
湖南中医药大学重点学科—基础医学 （）
湖南省大学生创新创业训练计划项目(编号:S202210541144). （编号:S202210541144）
