中药药理与临床2025，Vol.41Issue(1)：33-38,6.
基于肠道菌群-胆汁酸代谢途径探讨芩连红曲汤对高脂血症大鼠的改善作用
Qinlian Hongqu(芩连红曲)Decoction Ameliorates Hyperlipidemia in Rats via the Gut Microbiota-Bile Acid Metabolism Pathway
摘要
Abstract
Objective:To study the therapeutic effect of Qinlian Hongqu(芩连红曲)Decoction on hyperlipidemia and decipher the treatment mecha-nism.Methods:SD rats were randomly assigned into blank control and modeling groups,and those in the modeling group were fed with a high-fat diet for 12 weeks.The successfully modeled rats were randomly assigned into model control,Qinlian Hongqu Decoction(0.7 and 1.4 g/kg),and positive drug(atorvastatin,1.05 g/kg)groups,with 6 rats in each group.The changes in body weight before and after treatment were meas-ured.Biochemical methods were used to measure the levels of total cholesterol(TC),triglycerides(TG),and low-density lipoprotein-cholesterol(LDL-C)in the serum as well as the levels of TC and TG in the liver.Hematoxylin-eosin(HE)staining and oil red O staining were employed to observe the pathological changes and lipid deposition,respectively,in the liver.The 16S rRNA sequencing was employed to analyze the gut microbiota of rats,and targeted bile acid metabolomics was employed to observe the changes of bile acid metabolites in the gut contents of rats.Results:Compared with the blank control group,the model control group showed increased body weight,elevated levels of TC,TG,and LDL-C in the serum and TC,TG,and free fatty acids(FFA)in the liver,steatosis,and a large number of lipid droplets in the liver tissue.Compared with the model control group,Qinlian Hongqu Decoction reduced the body weight,blood lipid levels,and lipid deposition and lipid droplets in the liv-er.The gut microbiota structure showed significant differences between groups,with Firmicutes,Bacteroidetes,and Proteobacteria being domi-nant.Compared with the normal control group,the model control group showed increased relative abundance of Firmicutes and decreased relative abundance of Bacteroidetes.Compared with the model control group,Qinlian Hongqu Decoction(1.4 g/kg)decreased the relative abundance of Firmicutes and increased the relative abundance of Bacteroidetes,reversing the Firmicutes/Bacteroidetes(F/B)ratio.The targeted metabonom-ics of gut contents showed that Qinlian Hongqu Decoction promoted the excretion of bile acids.Conclusion:Qinlian Hongqu Decoction can a-meliorate hyperlipidemia in rats by regulating the gut microbiota-bile acid metabolism pathway.关键词
芩连红曲汤/高脂血症/肠道菌群/胆汁酸Key words
Qinlian Hongqu(芩连红曲)Decoction/Hyperlipidemia/Gut microbiota/Bile acid引用本文复制引用
杨炀,张永,赵美,罗雅琪,郭志清,张赣,何蕴良,陈东辉,张钟艺,张树森..基于肠道菌群-胆汁酸代谢途径探讨芩连红曲汤对高脂血症大鼠的改善作用[J].中药药理与临床,2025,41(1):33-38,6.基金项目
四川省科技厅中央引领地方项目(编号:2023ZYD0049) （编号:2023ZYD0049）
四川省中医药管理局中医药专项课题(编号:2023MS191) （编号:2023MS191）
四川省科技厅省级科研院所基本科研业务费项目(编号:2022JDKY0029). （编号:2022JDKY0029）
