中药药理与临床2025，Vol.41Issue(1)：58-63,6.
冠心舒通胶囊在脑损伤大鼠血浆、脑组织中的成分分析
Chemical Components of Guanxinshutong(冠心舒通)Capsules in Plasma and Brain Tissue of Rat Model of Cerebral Ischemia-Reperfusion Injury
摘要
Abstract
Objective:To qualitatively analyze the chemical components of Guanxinshutong(冠心舒通)Capsules(GXSTC)in the plasma and brain tissue of the rat model of cerebral ischemia-reperfusion injury.Methods:The rat model of MCAO/R was established by the suture meth-od.After successful modeling,the rats were randomized into middle cerebral artery occlusion/reperfusion(MCAO/R),MCAO/R+GXSTC(4 g/kg)groups,normal rats were served as sham operation,sham operation+GXSTC(4 g/kg)groups.The MCAO/R and sham operation groups received saline,and the MCAO/R+GXSTC(4 g/kg)and sham operation+GXSTC(4 g/kg)groups were administrated with GXSTC twice a day.After 5 consecutive days of drug administration by gavage,blood and cerebral cortex samples were collected from each group,and then the chemical components in the plasma and brain tissue were detected by UPLC/Q-TOF MS and GC-MS,respectively.Results:Com-pared with the sham operation group,the MCAO/R group showed increased neurological deficit score,cerebral infarction volume,and water content rate in the brain(P<0.01),which suggested that the model was successfully established.Compared with those in the MCAO/R group,the neurological deficit score,cerebral infarction volume,and water content rate in the brain reduced in the MCAO/R+GXSTC group(P<0.05 or P<0.01).In the two groups with GXSTC,10 major components of GXSTC were detected in rat plasma.Among them,ellagic acid,salvianolic acid B,eugenol,and borneol were detected in the brain tissue.Compared with the sham operation+GXSTC group,the MCAO/R+GXSTC group showcased decreased peak areas of components in the plasma and similar or mildly increased content of components in the brain.Conclusions:Ellagic acid,salvianolic acid B,eugenol,and borneol may be the key pharmacodynamic components of GXSTC in treating cerebral ischemia-reperfusion injury.关键词
冠心舒通胶囊/超高效液相色谱-电喷雾-四极杆-飞行时间-串联质谱技术/气相色谱-质谱联用技术/缺血性脑损伤/血浆/脑组织/鞣花酸/丹酚酸B/丁香酚/龙脑Key words
Guanxinshutong(冠心舒通)Capsules/UPLC/Q-TOF/MS/GC-MS/Cerebral ischemia-reperfusion injury/Plasma/Brain tissue/Ellagic acid/Salvianolic acid B/Eugenol/Borneol引用本文复制引用
张娟利,赵佳鑫,黄少杰,张迪,王婧雯,史明,文爱东..冠心舒通胶囊在脑损伤大鼠血浆、脑组织中的成分分析[J].中药药理与临床,2025,41(1):58-63,6.基金项目
国家自然科学基金面上项目(编号:82074321、82071464). （编号:82074321、82071464）
