中医药学报2025，Vol.53Issue(3)：19-23,5.DOI:10.19664/j.cnki.1002-2392.250047
麻杏石甘汤对慢性阻塞性肺疾病大鼠Th17/Treg平衡及肺功能的影响
Effects of Ephedra,Apricot Kernel,Gypsum and Licorice Decoction on Th17/Treg Balance and Pulmonary Function in Rats with Chronic Obstructive Pulmonary Disease
摘要
Abstract
Objective:To investigate the effects of Ephedra,Apricot Kernel,Gypsum and Licorice Decoction on the Th17/Treg balance and pulmonary function in rats with chronic obstructive pulmonary disease(COPD).Methods:A total of 80 healthy male rats were randomly divided into four groups:control group(Group A),model group(Group B),budesonide suspension group(Group C),and Ephedra,Apricot Kernel,Gypsum and Licorice Decoction combined with budesonide group(Group D),with 20 rats in each group.Except for Group A,the other three groups were used to establish the COPD model via smoke exposure combined with papain aerosol inhalation.After successful modeling,Group B was treated with physiological saline via gavage,Group C received budesonide suspension inhalation therapy,and Group D was given Ephedra,Apricot Kernel,Gypsum and Licorice Decoction in addition to budesonide suspension.Treatment was administered continuously for 60 days.HE staining was used to observe lung tissue pathology;flow cytometry was employed to measure the Th17/Treg balance in peripheral blood;oxidative stress levels in lung tissues were assessed using microplate methods;respiratory parameters and pulmonary function indices were detected with a needle electrode and biosignal acquisition system.Results:Compared with the control group,the alveolar structure was damaged in the model group,budesonide suspension group,and Ephedra,Apricot Kernel,Gypsum and Licorice Decoction combined with budesonide group,with the model group showing the most severe damage.The Th17/Treg ratio was higher and Treg cells were lower in the three treated groups compared to the control group,with significant differences(P<0.05).Among the treated groups,the Ephedra,Apricot Kernel,Gypsum and Licorice Decoction combined with budesonide group showed significant improvement compared to the budesonide suspension group(P<0.05).Compared to the control group,the activities of SOD and GSH-Px in lung tissues decreased,and MDA levels increased in the three treated groups,with significant differences(P<0.05).The Ephedra,Apricot Kernel,Gypsum and Licorice Decoction combined with budesonide group showed significant improvement compared to the budesonide suspension group(P<0.05).Respiratory function was significantly impaired in the three treated groups compared to the control group(P<0.05),with the Ephedra,Apricot Kernel,Gypsum and Licorice Decoction combined with budesonide group showing the most notable improvement compared to the budesonide suspension group(P<0.05).Compared with the control group,the peak inspiratory flow(PIF),peak expiratory flow(PEF),and minute ventilation(MV)were reduced in the three treated groups(P<0.05),with the Ephedra,Apricot Kernel,Gypsum and Licorice Decoction combined with budesonide group demonstrating more significant improvements compared to the budesonide suspension group(P<0.05).Conclusion:Ephedra,Apricot Kernel,Gypsum and Licorice Decoction can improve lung tissue pathological damage in COPD rats,promote recovery of the Th17/Treg balance,enhance respiratory function,and improve pulmonary function.关键词
麻杏石甘汤/慢性阻塞性肺疾病/辅助性T细胞/调节性T细胞/肺功能Key words
Ephedra,Apricot Kernel,Gypsum and Licorice Decoction/Chronic obstructive pulmonary disease/Thelper cells/Regulatory T cells/Pulmonary function分类
中医学引用本文复制引用
苏君毅,刘海军..麻杏石甘汤对慢性阻塞性肺疾病大鼠Th17/Treg平衡及肺功能的影响[J].中医药学报,2025,53(3):19-23,5.基金项目
河南省高等学校重点科研项目计划(20A310007,21B360002) （20A310007,21B360002）
