中医药学报2025，Vol.53Issue(3)：24-31,8.DOI:10.19664/j.cnki.1002-2392.250048
三七总皂苷乳膏对皮肤缺损模型小鼠创面愈合的影响
Effects of Panax Notoginseng Total Saponins Ointment on Wound Healing in a Skin Defect Model of Mice
摘要
Abstract
Objective:To investigate the therapeutic effects of Panax Notoginseng total saponins ointment on skin defect models in mice.Methods:Sixty SPF-grade healthy female mice were randomly divided into the model group,positive control group(recombinant human epidermal growth factor gel),blank ointment matrix group,and Panax Notoginseng ointment group,with 15 mice in each group.A full-thickness skin defect model was established by excising a 1 cm circular piece of skin down to the fascia along the midline of the mice's backs.After 2 hours of modeling,treatments were applied,with medication replaced once daily for 21 consecutive days.Wound healing was observed,and the healing rates were calculated on days 3,7,and 14 after modeling.Hematoxylin-eosin(HE)and Masson staining were performed to observe the pathological changes of the wound.Enzyme-linked immunosorbent assay(ELISA)was used to detect serum levels of TNF-α,IL-6,VEGFA,STAT3,and HSP90AA1.Results:Over time,significant changes in the wound were observed in both the Panax Notoginseng ointment group and the positive control group,with a notably faster healing rate(P<0.05).HE and Masson staining results showed that the proliferation of inflammatory cells was significantly reduced in the Panax Notoginseng ointment group and the positive control group compared to the other two groups(P<0.05).The number of new capillaries and fibroblasts in the wound tissue increased to varying extents in all groups,especially in the Panax Notoginseng ointment group and the positive control group,with a significant increase(P<0.05).Panax Notoginseng ointment reduced the levels of inflammatory factors IL-6 and TNF-α in the serum(P<0.05)and modulated the expression of STAT3 protein in the skin tissue,upregulating the expression levels of VEGFA and HSP90AA1(P<0.05).Conclusion:Panax Notoginseng total saponins promote skin wound healing in a full-thickness skin defect model in mice.关键词
三七总皂苷/皮肤损伤/创面愈合Key words
Panax notoginseng total saponins/Skin injury/Wound healing分类
中医学引用本文复制引用
代思思,崔秀秀,黄敬文..三七总皂苷乳膏对皮肤缺损模型小鼠创面愈合的影响[J].中医药学报,2025,53(3):24-31,8.基金项目
黑龙江省教育厅科研项目(12541765) （12541765）
黑龙江省博士后科研启动资助项目(LBH-Q18126) （LBH-Q18126）
