中医药学报2025，Vol.53Issue(3)：37-42,6.DOI:10.19664/j.cnki.1002-2392.250050
野菊花HPLC指纹图谱及化学模式识别的研究
Study on the HPLC Fingerprint and Chemical Pattern Recognition of Wild Chrysanthemum
摘要
Abstract
Objective:To establish the High-Performance Liquid Chromatography(HPLC)fingerprint of wild chrysanthemum and evaluate the quality of wild chrysanthemum from different origins and batches using chemical pattern recognition.Methods:The study utilized a Waters Symmetry C18 column(4.6 mm×250 mm,5 μm)with a mobile phase of acetonitrile-0.1%phosphoric acid aqueous solution for gradient elution.The flow rate was set at 0.6 mL·min-1,the column temperature at 25℃,and the UV detection wavelength at 334 nm,with an injection volume of 10 μL.Various chemical pattern recognition methods were used for the overall quality evaluation of multiple batches of wild chrysanthemum.Results:The HPLC fingerprints of 15 batches of wild chrysanthemum identified 18 common peaks,with similarities all greater than 0.9.Eleven common peaks,including linarin and neochlorogenic acid,were identified.Using Hierarchical Cluster Analysis(HCA),Principal Component Analysis(PCA),and Orthogonal Partial Least Squares-Discriminant Analysis(OPLS-DA),the 15 batches of wild chrysanthemum were categorized into three major groups,identifying two principal components and three differential quality markers:isochlorogenic acid A,isochlorogenic acid C,and linarin,which can be used as quality control indicators for wild chrysanthemum.Conclusion:The successfully established HPLC fingerprint method for wild chrysanthemum is simple,accurate,and has good reproducibility and reliability.It provides a reliable reference for the quality control and clinical application of wild chrysanthemum medicinal materials and preparations.关键词
野菊花/高效液相色谱指纹图谱/质量评价/化学模式识别Key words
Wild chrysanthemum/HPLC fingerprint/Quality evaluation/Chemical pattern recognition分类
医药卫生引用本文复制引用
吴金富,吕涵,金阳,王艳艳,郭勇,李丹,黄莉莉..野菊花HPLC指纹图谱及化学模式识别的研究[J].中医药学报,2025,53(3):37-42,6.基金项目
国家自然科学基金项目(82003974) （82003974）
黑龙江省博士后科研启动金(LBH-Q13161) （LBH-Q13161）
黑龙江省自然科学基金(LH2019h106) （LH2019h106）
黑龙江中医药中青年科技攻关项目(ZQG-042) （ZQG-042）
