中医药学报2025，Vol.53Issue(3)：43-51,9.DOI:10.19664/j.cnki.1002-2392.250051
复方刺五加颗粒HPLC指纹图谱建立及多成分含量测定研究
Establishment of HPLC Fingerprint and Multi-Component Content Determination of Compound Eleutherococcus Senticosus Granules
摘要
Abstract
Objective:To establish the HPLC fingerprint of Compound Eleutherococcus Senticosus Granules and to determine the content of six components,providing a reference for quality control and evaluation.Methods:HPLC was used to establish the fingerprint of Compound Eleutherococcus Senticosus Granules from 10 batches,followed by similarity evaluation and chemical pattern recognition analysis.The contents of the six components in the granules,namely,Syringin,Eleutheroside E,Isohypaphorine,Schisandrin A,Schisandrin B,and Schisandrin C,were also measured.Results:The similarity of the fingerprints from the 10 batches was all>0.921.The linear ranges for Syringin,Eleutheroside E,Isohypaphorine,Schisandrin A,Schisandrin B,and Schisandrin C were 2.92～5.84 μg,2.55～5.10 μg,0.405～0.81 μg,0.095～0.19 μg,0.14～0.28 μg,and 0.68～1.36 μg,respectively,all showing good linear relationships.The recovery rates were 100.2%～101.4%,with RSDs of 1.2%～1.6%.Precision,repeatability,and stability tests all met the required standards.Conclusion:The established method is simple and convenient,providing a basis for evaluating the quality of Compound Eleutherococcus Senticosus Granules.关键词
复方刺五加颗粒/含量测定/指纹图谱Key words
Compound eleutherococcus senticosus granules/Content determination/Fingerprint分类
医药卫生引用本文复制引用
杨雯雯,杨赛赛,王旭,严妍,刘磊,吴修红..复方刺五加颗粒HPLC指纹图谱建立及多成分含量测定研究[J].中医药学报,2025,53(3):43-51,9.基金项目
黑龙江省揭榜挂帅(2023ZXJ02C01) （2023ZXJ02C01）
黑龙江省重点研发计划(2024ZX12C16) （2024ZX12C16）
