中华中医药学刊2025，Vol.43Issue(2)：25-28,后插3-后插6,8.DOI:10.13193/j.issn.1673-7717.2025.02.005
基于SDF-1/CXCR4轴探讨雷公藤多糖诱导胰腺干细胞分化的机制研究
Exploring Mechanism of Tripterygium Wilfordii Polysaccharides Inducing Pancreatic Stem Cell Differentiation Based on SDF-1/CXCR4 Axis
摘要
Abstract
Objective To investigate the mechanism of Tripterygium wilfordii polysaccharides inducing pancreatic stem cell dif-ferentiation through stromal cell-derived factor-1(SDF-1)/CXC chemokine receptor-4(CXCR4)axis.Methods Primary pancreatic stem cells were prepared from pancreatic tissue from Kunming mice on day 4 of neonatal birth.The immunohistochem-istry method was used to identify the pancreatic stem cells.Islet β cells were identified by di thiazone(DTZ)staining.An invert-ed fluorescence microscope was used to photograph and record the cells at various cultivation time points.The release of SDF-1 from cells were detected at various administration time points by using enzyme-linked immunosorbent assay(ELISA).The rel-ative mRNA expression levels of SDF-1 and CXCR4 in each group were detected by real-time fluorescence quantitative PCR(qPCR)technology.The expression levels of SDF-1 and CXCR4 proteins in each group were detected by Western blot assay(WB).Results The cells obtained was Nestin positive and Brownish-yellow were identified by immunohistochemistry method.DTZ staining was positive,and the number of positive stained cells in the treatment group was significantly higher than that in the control group(P＜0.05).The release of SDF-1 from the control group cells remained relatively stable,while the release of SDF-1 from the experimental group cells gradually increased over time and reached its peak on the 26th day.Subsequently,the release of SDF-1 gradually decreased over time.The relative mRNA expression levels of SDF-1 and CXCR4 in the experimen-tal group cells were significantly higher than those in the control group cells detected by qPCR technology(P＜0.05).The ex-pression levels of SDF-1 and CXCR4 proteins in the experimental group cells were significantly higher than those in the control group cells by WB(P＜0.05).Conclusion Tripterygium wilfordii polysaccharides can induce pancreatic stem cell differentiation through the SDF-1/CXCR4 axis.关键词
雷公藤多糖/胰腺干细胞/基质细胞衍生因子-1/CXC型趋化因子受体4Key words
Tripterygium wilfordii polysaccharides/pancreatic stem cell/stromal cell derived factor-1/CXC chemokine re-ceptor-4分类
医药卫生引用本文复制引用
贾微,宁志莹,岑妍慧,刘鑫,杨瑞,李博佳,李森,张宸康..基于SDF-1/CXCR4轴探讨雷公藤多糖诱导胰腺干细胞分化的机制研究[J].中华中医药学刊,2025,43(2):25-28,后插3-后插6,8.基金项目
国家自然科学基金项目(82260861) （82260861）
广西一流学科建设开放课题(2019XK137) （2019XK137）
广西一流学科建设项目重点课题(2018XK023,2018XK024) （2018XK023,2018XK024）
广西高校中青年教师科研基础能力提升项目(2021KY0291) （2021KY0291）
广西中医药大学桂派杏林拔尖人才项目(2022C002) （2022C002）
广西中医药大学桂派中医药传承创新团队资助项目(2022B004) （2022B004）
广西中医药大学校级重点硕士研究生科研创新项目(YCSZ2022003) （YCSZ2022003）
广西中医药大学大学生创新创业训练计划项目(202210600017,202010600018) （202210600017,202010600018）
广西中医药大学校级科研项目(2019MS001) （2019MS001）
