草食家畜Issue(1)：15-22,8.DOI:10.16863/j.cnki.1003-6377.2025.01.003
长期高铜饲喂对绵羊胰腺损伤的影响
Long-term High Copper Feeding Causes Pancreatic Injury in Sheep Through Pyroptosis
摘要
Abstract
[Objective]The aim is to explore the effects of long-term high-copper feeding on the histological damage of the pancreas in sheep,so as to provide a theoretical basis for the scientific and reasonable addition of copper in feed,and to provide a research basis of molecular pathology for clinical copper poisoning.[Methods]Eight healthy 12-month-old Northeast fine wool sheep were selected and randomly divided into two groups,including the control group in which four sheep were fed with normal diet,and the experimental group in which four sheep were fed with high copper(Cu 20 mg/kg)based on normal diet.The gavage time of high copper was once each in the morning and afternoon.After 8 weeks,sheep pancreatic tissues of the control and experimental groups were collected and put into Bouin fixative,2.5%glutaraldehyde,or liquid nitrogen.The pathological changes in pancreatic tissues were observed by transmission electron microscopy after hematoxylin-eosin(H.E.)staining of pathological sections,the diameter and area of the pancreatic acinar cells were measured,and the expression levels of pyroptosis proteins such as Caspase-1,GSDMD and IL-18 in pancreatic tissues were detected by immunofluorescence and immunohistochemistry methods.[Results]The results showed that compared with the control group,the pancreatic tissue of the experimental group swelled obviously and the diameter and area of the acinar cells were significantly larger.Ultrastructure analysis showed that the endoplasmic reticulum and mitochondria of pancreatic acinar cells in the experimental group were damaged,which was consistent with the ultrastructural characteristics of pyroptosis.The expression levels of Caspase-1,GSDMD and IL-18 in pancreatic tissue of the experimental group were higher than those of the control group.[Conclusion]In conclusion,high copper feeding to sheep would damage pancreatic tissue through pyroptosis.Data support was provided by this study for revealing the molecular mechanism of sheep pancreatic injury by high copper feeding,and theoretical reference for scientific and reasonable addition of copper in feed,as well as molecular pathology research basis for clinical copper poisoning.关键词
铜/绵羊/胰腺/细胞焦亡/组织学Key words
copper/sheep/pancreas/pyroptosis/histology分类
农业科技引用本文复制引用
康馨月,吴君仰,平悦,周慧敏,滕春波,王春生..长期高铜饲喂对绵羊胰腺损伤的影响[J].草食家畜,2025,(1):15-22,8.基金项目
国家自然科学基金面上项目(32072801) （32072801）
黑龙江省大学生创新创业训练计划项目(S202210225323,DC-2023192) （S202210225323,DC-2023192）
