广东农业科学2025，Vol.52Issue(1)：1-13,13.DOI:10.16768/j.issn.1004-874X.2025.01.001
云南三地布朗族聚集区地方稻种资源苗瘟抗性评价及抗性基因鉴定
Evaluation of Seedling Blast Resistance and Identification of Resistance Genes in Rice Landraces from Three Bulang Minority Gathering Areas inYunnan
摘要
Abstract
[Objective]In order to determine the resistance characteristics and distribution of rice blast disease in rice landraces from Bulang minority in Yunnan.[Method]Seedling-stage resistance evaluation and molecular characterization were conducted on 78 rice landraces using 31 functional markers targeting 24 blast resistance genes and 10 blast fungal strains.[Result]Inoculation assays revealed that the 78 rice landraces exhibited resistance levels ranging from 40%to 100%,with an average resistance rate of 93.6%.Notably,55 accessions(70.5%)demonstrated complete resistance(100%).Regional resistance patterns were observed as follows:Menghai County(99.00%)>Mojiang County(89.09%)>Shuangjiang County(80.59%).The resultes of Pathogen Virulence Profiling showed strains 08-16-6a-1 and HN-09-1c-7 displayed the highest virulence against Bulang landraces(11.54%susceptibility),where Menghai accessions being most susceptible to strains HN-09-1c-7 and 08-20-1a-1(4.00%susceptibility),Mojiang landraces showed 36.36%susceptibility to strain 08-20-1a-1,Shuangjiang resources exhibited the highest susceptibility(52.94%)to strain 08-16-6a-1.Functional gene analysis revealed the presence of six blast resistance genes(Pi9,Pi5,Pita,Pikh,Pid2,and Pikm)in the Bulang rice landraces,with allele frequencies ranging from 73.08%to 84.62%.The Pid2 exhibited the highest overall frequency,while Pi9 showed the lowest.Regional variations were observed:In Menghai County,Pita and Pid2 predominated(84.62%),contrasting with the reduced prevalence of Pi9(73.08%).Mojiang County displayed elevated frequencies of Pid2,Pi5,and Pi9(84.62%),whereas Pita was least frequent(73.08%).In Shuangjiang County,Pid2,Pita,Pikm,Pikh,and Pi9 were dominant(84.62%),while Pi5 occurred at the lowest frequency(73.08%).Regression analysis demonstrated no significant correlations(P>0.05)between these six genes and seedling-stage leaf blast resistance.Gene combinations accounted for 1.8%to 25.8%of resistance variability,with the Pid2+Pi5+Pikm+Pikh quartet showing the lowest explanatory power(1.8%)and the six-gene combination exhibiting the highest contribution(25.8%).However,no statistically significant synergistic effects were observed among the combined genes.[Conclusion]The Bulang rice landraces of Yunnan possess robust and broad-spectrum blast resistance,predominantly mediated by the Pid2 gene.Thirty-eight accessions with complete resistance(100%)to 10 strains and co-occurrence of Pid2 and Pi9 alleles were identified.These accessions represent valuable genetic resources for diversifying the genetic basis of blast resistance in modern rice breeding programs.关键词
地方稻种资源/布朗族/稻瘟病/抗性基因/功能标记/致病力Key words
rice landraces/Bulang minority/rice blast disease/resistance gene/functional marker/pathogenicity分类
农业科技引用本文复制引用
董超,董丽英,薛静,杨雅云,汤翠凤,阿新祥,张斐斐,杨勤忠,戴陆园..云南三地布朗族聚集区地方稻种资源苗瘟抗性评价及抗性基因鉴定[J].广东农业科学,2025,52(1):1-13,13.基金项目
云南省重大科技专项(202302AE090011) （202302AE090011）
云南省科技人才与平台计划项目(202405AD350066) （202405AD350066）
国家重点研发计划子课题项目(2021YFD1200505-1) （2021YFD1200505-1）
云南省种子种业联合实验室项目(202205AR070001-11) （202205AR070001-11）
