干旱区研究2025，Vol.42Issue(3)：499-510,12.DOI:10.13866/j.azr.2025.03.10
基于SSR分子标记的5个黄花补血草居群花型变异及其遗传效应
Floral morph variation and genetic effect of five Limonium aureum populations based on Simple Sequence Repeat molecular markers
摘要
Abstract
In heterostylous plants,the floral morph composition and frequency of populations are a consequence of mating events over generations."Homostyly"with anthers and stigmas at the same level within a flower is a floral morph that frequently appears in the evolution of heterostyly and is often accompanied by breakdown of the heteromorphic incompatibility system and the decline of genetic diversity.To explore the formation of the H-morph and its effects on the population,we studied five Limonium aureum populations exhibiting a widely distrib-uted floral morph(H-morph),similar to"homostyly,"in the southern margin of Tarim Basin.The floral morph composition and heterostylous syndrome were investigated by field observations and hand pollination experi-ments.Meanwhile,the genetic diversity and genetic structure were studied using SSR molecular markers.The re-sults showed the following:(1)The Atushi(ATS)population consisted only of the H-morph,while the remaining four populations were composed of the long-and short-styled morph,and H-morph.All populations had a moder-ate level of genetic diversity,dimorphism of stigma-pollen morphology,and a strict heteromorphic incompatibili-ty system,revealing that the floral morphs with different pollen ornamentation and stigma papilla cell morpholo-gy were compatible.(2)Genetic structure analysis,principal coordinate analysis,and phylogenetic analysis showed that the ATS population had emerged earlier and was independent of the other four populations phyloge-netically,with a significant correlation between genetic distance and geographic distance.(3)The H-morphs of the two types of populations may be at different stages of the evolution of heterostyly.The self-incompatibility of the H-morph and the heteromorphic incompatibility system of the population maintained the population's genetic diversity.关键词
异型花柱/黄花补血草/花型变异/遗传多样性/同长花柱Key words
heterostyly/Limonium aureum/floral variation/genetic diversity/homostyly引用本文复制引用
张晶,何爽,张爱勤..基于SSR分子标记的5个黄花补血草居群花型变异及其遗传效应[J].干旱区研究,2025,42(3):499-510,12.基金项目
国家自然科学基金项目(32360308) （32360308）
自治区教育厅项目(XJEDU2021I006) （XJEDU2021I006）
