西部中医药2025，Vol.38Issue(3)：1-9,9.DOI:10.12174/j.issn.2096-9600.2025.03.01
基于网络药理学与"以药测证"挖掘非酒精性脂肪性肝病的证型、治法与方药
The Syndromes,Therapeutic Method and Prescriptions of Nonalcoholic Fatty Liver Disease Based on Network Pharmacology and"Syndrome Differentiation by Effect of Drugs"
摘要
Abstract
Objective:To survey the pathogenesis,therapeutic methods and prescriptions of nonalcoholic fatty liver disease(NAFLD),and to verify the effectiveness of the predicted Chinese medicine compound.Methods:Protein-protein interaction(PPI),topology analysis and reversed herbal targets established by therapeutic targets database(TTD),were explored to discuss the pathogenesis and prescriptions of NAFLD.Male adult Wistar rats were employed as the subjects in the experiment,all 24 Wistar rats were allocated to the blank group,HFD group and herb group(n=8),the predicted therapeutic effects of the prescription on NAFLD were assessed by detecting body mass,liver weight,liver index,visceral fat mass,body fat percentage and serum biochemical examination.Results:Reverse drug target network was adopted to predict and analyze 14 syndromes of NAFLD,Sangye(Mori folium),Jinyinhua(Lonicerae japonicae flos),Shaji(Hippophae fructus),Yinxingye(Ginkgo Folium)and Gehua(Flower of lobed Kudzuvine)were used as the based prescription,16 herbal categories were consulted and chosen according to the symptoms,the experiments displayed that the predicted prescription could noticeably improve the pathology of NAFLD.When HFD group was compared with the blank group,body mass,liver weight,liver index,visceral fat mass,body fat percentage,and the contents of AST,ALT,TG,TC,FFA and TG in liver tissue were elevated(P<0.01),the decrease in body mass,liver weight,liver index,visceral fat mass,body fat percentage,and the contents of AST,ALT,TG,TC,FFA and TG in liver tissue was more evident in the herb group compared with HFD group(P<0.05);the content of TG was higher in HFD group than that of the blank group(P<0.01),pathological section showed significant balloon-like changes,inflammatory cell aggregation and partial hepatocyte necrosis in the liver.Conclusion:NAFLD is mainly related to heat,toxin,wind,phlegm,stasis and dampness,involving 14 various syndromes,treated with the methods of clearing heat,detoxifying,resolving phlegm,relieving pain,invigorating spleen and dispelling wind,and animal experiments have been conducted to verify the therapeutic effects of Sangye,Jinyinhua,Shaji,ginkgo and Gehua as the base prescription for NAFLD.关键词
非酒精性脂肪性肝病/网络药理学/反向药靶/以药测证Key words
nonalcoholic fatty liver disease/network pharmacology/reversed herbal targets/syndrome differentiation by effect of drug分类
医药卫生引用本文复制引用
穆杰,鲁法庭,王东,苏悦,刘西洋,冯全生..基于网络药理学与"以药测证"挖掘非酒精性脂肪性肝病的证型、治法与方药[J].西部中医药,2025,38(3):1-9,9.基金项目
四川省青年基金项目(2024NSFSC1829,2025ZNSFSC1795) （2024NSFSC1829,2025ZNSFSC1795）
成都市卫生健康委员会联合创新专项(WXLH202402052) （WXLH202402052）
成都中医药大学青基人才专项(QJRC2024002). （QJRC2024002）
