摘要
Abstract
Objective:To provide science-based theoretical reference for"the same treatment for different diseases"in TCM by discussing the mechanism of Sanren Tang in the treatment of PCOS and type 2 diabetes mellitus(T2DM)based on network pharmacology.Methods:The platforms including TCMSP,TCMID,and TCMIP,HIT2.0,BATMAN-TCM,and SymMap,were applied to obtain and screen out the active ingredients of Sanren Tang,and to judge its biological activity;The target genes corresponding to the effective active components were obtained,entered into the Uniprot database to standardize the targets;GeneCards,TTD,CTD and DrugBank were utilized to obain the targets of PCOS and T2DM,and the common targets of the decoction in the treatment of PCOS and T2DM;Cytoscape 3.7.1 software was adopted to draw the network of"single herb-active ingredient-common targets"of"the same treatment for different diseases"in Sanren Tang;STRING was used to map protein interaction network;biological processes were conducted through DAVID,and R studio to perform Mode analysis,GO enrichment analysis and KEGG enrichment analysis.Results:The study has yielded 50 effective active ingredients of the decoction,23 332 targets of PCOS,28 438 targets of T2DM,and 214 targets of the treatment of PCOS and T2DM with Sanren Tang on the foundation of the same treatment for different diseases;39 key targets were identified for PPI;the results of GO and KEGG enrichment analysis showed that"the same treatment for different diseases"of Sanren Tang mainly involved inflammatory reaction,hypoxia reaction,apoptosis,HIF-1 signaling pathway,calcium ion channel and insulin resistance(IR);the results of MCODE analysis displayed that"the same treatment for different diseases"of Sanren Tang might be related to reactive oxygen species(ROS)response,apoptosis-related signaling pathway,IL-17 signaling pathway and fatty acid metabolism processes;the key targets of"the same treatment for different diseases"of Sanren Tang contained AKT1,TP53,CASP3,TNF and IL-6.Conclusion:Sanren Tang could develop the effects in the treatment of PCOS and T2DM possibly through the mechanism of multi-target,multi-pathway and multi-way,so as to achieve the effects of treating different diseases with the same therapy.关键词
多囊卵巢综合征/2型糖尿病/三仁汤/异病同治Key words
PCOS/type 2 diabetes mellitus/Sanren Tang/the same treatment for different diseases分类
医药卫生
