国际老年医学杂志2025，Vol.46Issue(2)：201-206,6.DOI:10.3969/j.issn.1674-7593.2025.02.013
低频超声联合纳米微泡对老年脑血栓患者治疗效果评价及血清标志物相互作用的网络构建
Evaluation of the therapeutic effect of low-frequency ultrasound combined with nano-microbubbles on the treatment of elderly patients with cerebral thrombosis and the construction of serum marker interaction network
摘要
Abstract
Objective To investigate the effect of low-frequency ultrasound combined with nano-microbubbles therapy on neurological,motor function and serological marker levels in elderly patients with cerebral thrombosis,and to construct a protein-pro-tein interaction(PPI)network based on serological markers.Methods ①A total of 136 elderly patients with cerebral thrombosis treated in Xi'an International Medical Center Hospital from March 2022 to September 2023 were selected.The patients were divided in-to observation group and control group by random number table,each had 68 cases.Both groups were treated with intravenous throm-bolysis,i.e.Patients in observation group were treated with low-frequency ultrasound combined with nano-microbubbles therapy.Ser-um marker levels,coagulation function,nerve and motor function were compared between the two groups.②The PPI network was con-structed using the String database.The gene ontology(GO)function enrichment analysis and the Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis were performed using R software.Results ①After treatment,6 minute walking distanc,Bar-thel index score,upper limb Fugl-Meyer assessment(FMA)score and lower limb FMA score in observation group were higher than those in control group(P<0.05),and National Institute of Health Stroke Scale in observation group was lower than that in control group(P<0.05).After treatment,the serum levels of vascular endothelial growth factor(VEGF),S100 calcium binding protein B(S100β),nerve growth factor(NGF),Brain derived neurotrophic factor(BDNF)and neurotrophin 3(NT-3)in observation group were higher than those in control group(P<0.05).The serum S100β level in observation group was lower than that in control group(P<0.05).After treatment,there was no significant difference in prothrombin time,activated partial thromboplastin time,thrombin time between the two groups(P>0.05).②VEGF,NGF,BDNF,NT-3 and S100β have extensive protein-protein interactions.PPI network node genes were significantly enriched in KEGG pathways such as Ras signaling pathway,neurotrophic factor signaling pathway and Rap1 signaling pathway,and significantly enriched in GO functional items such as denating-membrane receptor protein tyrosine ki-nase signaling pathway,neurotrophic binding,and axons.Conclusion Low-frequency ultrasound combined with nano-microbubbles can promote the recovery of nerve function,increase the level of neurotrophic factor and reduce the degree of brain injury in elderly pa-tients with cerebral thrombosis.The potential mechanisms may include the Ras signaling pathway,neurotrophic factor signaling pathway and Rap1 signaling pathway.关键词
脑血栓/低频超声/纳米微泡Key words
Cerebral thrombosis/Low-frequency ultrasound/Nano-microbubbles引用本文复制引用
陈姗姗,周晓东..低频超声联合纳米微泡对老年脑血栓患者治疗效果评价及血清标志物相互作用的网络构建[J].国际老年医学杂志,2025,46(2):201-206,6.基金项目
陕西省重点研发计划项目(2023-YBSF-121) （2023-YBSF-121）
陕西省自然科学基础研究计划项目(2023-JC-QN-0912) （2023-JC-QN-0912）
