医学分子生物学杂志2025，Vol.22Issue(2)：101-107,7.DOI:10.3870/j.issn.1672-8009.2025.02.001
miR-490-5p调节低氧诱导的喉癌FaDu细胞炎症反应和血管生成的体外研究
In Vitro Study of miR-490-5p Regulating Inflammation and Angiogen-esis in Hypoxy-induced Laryngeal Carcinoma FaDu Cells
摘要
Abstract
Objective To investigate the effect and molecular mechanism of microRNA-490-5p(miR-490-5p)on inflammatory response and angiogenesis in human laryngeal cancer cells induced by hypoxia.Methods The human hypopharyngeal squamous cell carcinoma cell line FaDu was di-vided into 4 groups:control group(cultured in normoxic environment),hypoxic group(cultured in hypoxic environment),hypoxic+NC group(cultured in hypoxic environment and then transfect-ed with mimics NC),and hypoxic+miR-490-5p group(cultured in hypoxic environment and then transfected with miR-490-5p mimics).The expression level of miR-490-5p in cells was detected by RT-qPCR,the cell proliferation activity was detected by CCK-8 method,the levels of tumor necro-sis factor-α(TNF-α),interleukin(IL)-1β and IL-6 in the cell supernatant were determined by ELISA,the expression levels of proteins related to the nuclear factor κB(NF-κB)pathway in cells was detected by Western blotting,the number of HUVEC tubes formed was detected by in vitro an-giogenesis assay,the expression levels of vascular endothelial growth factor(VEGF),epidermal growth factor(EGF)and hypoxia-inducible factor-1α(HIF-1α)in cells was detected by RT-qPCR and Western blotting.Results Compared with those in the control group,the relative ex-pression level of miR-490-5p in the hypoxia group was down-regulated(P<0.05),the prolifera-tive activity was increased(P<0.05),the levels of TNF-α,IL-1β and IL-6 in supernatant were increased(P<0.05),the relative expression levels of phosphorylated NF-κB P65(p-NF-κB P65)and phosphorylated IκB kinase α(p-IκBα)were up-regulated(P<0.05),and the num-ber of HUVEC tubes was increased(P<0.05),the mRNA and protein relative expression levels of VEGF,EGF and HIF-1α were up-regulated(P<0.05).Compared with those in the hypoxia+NC group,the relative expression level of miR-490-5p in the hypoxia+miR-490-5p group was up-regu-lated(P<0.05),the proliferation activity was decreased(P<0.05),and the levels of TNF-α,IL-1β and IL-6 in supernatant were decreased(P<0.05),the relative expression levels of p-NF-κB P65 and p-IκBα were down-regulated,while the number of HUVEC tube formation was de-creased(P<0.05),and the mRNA and protein expression levels of VEGF,EGF and HIF-1α were also down-regulated(P<0.05).Conclusion Under hypoxic conditions,overexpression of miR-490-5p can inhibit the inflammatory response of laryngeal cancer cells and reduce the formation of tumor blood vessels.关键词
喉癌/低氧/miR-490-5p/炎症/血管生成Key words
laryngocarcinoma/hypoxia/miR-490-5p/inflammation/angiogenesis分类
临床医学引用本文复制引用
谭元元,艾合买提江·艾力江,周慧银..miR-490-5p调节低氧诱导的喉癌FaDu细胞炎症反应和血管生成的体外研究[J].医学分子生物学杂志,2025,22(2):101-107,7.基金项目
新疆维吾尔自治区自然科学基金(No.2021D01C187) This work was supported by a grant from Xinjiang Uygur Autonomous Region Natural Science Foundation(No.2021D01C187) （No.2021D01C187）
