医学分子生物学杂志2025，Vol.22Issue(2)：108-116,9.DOI:10.3870/j.issn.1672-8009.2025.02.002
基于生物信息学分析结肠癌关键基因DHCR7及其功能验证
Bioinformatics Analysis of DHCR7 in Colon Cancer and Its Functional Validation
摘要
Abstract
Objective To explore the effects and regulatory mechanisms of dehydrocholesterol reductase 7(DHCR7)on the proliferation,apoptosis,migration,and invasion ability of colon cancer(CC)cells.Methods Using bioinformatics methods to analyze the relationship between DHCR7 expression and immune infiltration,prognosis,and sensitivity to anti-tumor drugs in colon cancer.qRT-PCR,Western blotting,and IHC were used to detect the mRNA and protein expres-sion levels of DHCR7.Effect of DHCR7 knock-down on cell proliferation,cell cycle and apoptosis,cell migration and invasion was determined by MTT and colony formation assay,flow cytometry,wound-healing and Transwell assay,respectively.The key signaling pathways regulated by DHCR7 were screened out through gene enrichment analysis and validated by Western blotting.Results Bioinformatics analysis shows that the high expression of DHCR7 in colon cancer tissues was associ-ated with the N-stage,specific survival,progression free survival,tumor immune infiltration level,and drug sensitivity of colon cancer.DHCR7 expression was upregulated in colon cancer tissues and cell lines.Knockdown of DHCR7 expression inhibited the proliferation,migration,and invasion of HCT166 cells.Wnt signaling pathway was one of the key signaling pathways regulated by DHCR7 in CC,and knockdown of DHCR7 inhibited Wnt signaling pathway.Conclusion DHCR7 regulates the Wnt signaling pathway to promote EMT in colon cancer cells,thereby promoting proliferation,migration,and invasion of HCT116 cells.关键词
结肠癌/脱氢胆固醇还原酶7/细胞增殖/细胞迁移/Wnt信号通路Key words
colon cancer/7-dehydrocholesterol reductase/cell proliferation/cell migration/Wnt signaling pathway分类
临床医学引用本文复制引用
白小岗,王延峰,刘永成,姬海涛,张静..基于生物信息学分析结肠癌关键基因DHCR7及其功能验证[J].医学分子生物学杂志,2025,22(2):108-116,9.基金项目
陕西省自然科学基础研究计划(No.2024JC-YBQN-0983),中国高校产学研创新基金-华通国康医学科研资助项目(No.2023HT056),延安市科技计划项目(No.2022SLSFGG-022). This work was supported by grants from Shaanxi Provincial Natural Science Basic Research Program(No.2024JC-YBQN-0983),China University Re-search Innovation Fund Huatong Guokang Medical Research Support Project(No.2023HT056),and Yan'an Science and Technology Plan Project(No.2022SLSFGG-022) （No.2024JC-YBQN-0983）
