医学分子生物学杂志2025，Vol.22Issue(2)：117-123,138,8.DOI:10.3870/j.issn.1672-8009.2025.02.003
LINC00365靶向调控miR-519d影响甲状腺癌细胞的增殖、凋亡、迁移与侵袭
Effect of LINC00365 on Proliferation,Apoptosis,Migration,and Invasion of Thyroid Cancer Cells by Targeting miR-519d
摘要
Abstract
Objective To investigate the effects and mechanisms of LINC00365 on prolifera-tion,apoptosis,migration and invasion of thyroid cancer cells.Methods qRT-PCR was used to detect the relative expression level of LINC00365 in thyroid cancer tissues and thyroid cancer cell lines.Thyroid cancer cells BHP-10-3 were divided into 5 groups:siNC group,si-LINC00365 group,si-LINC00365+NC inhibitor group,si-LINC00365+miR-519d inhibitor group,and blank group.Dual luciferase reporter gene experiment was used to detect the targeting relationship between LINC00365 and miR-519d.Cell proliferation ability was detected by CCK8,cell migration ability was detected by wound healing assay,cell invasion ability was detected by transwell assay,apopto-sis rate was detected by flow cytometry.Results The relative expression level of LINC00365 were significantly upregulated in thyroid cancer tissues and thyroid cancer cells.miR-519d is the target gene of LINC00365.the proliferation,migration,and invasion abilities of BHP-10-3 in the si-LINC00365 group and the si-LINC00365+NC inhibitor group were significantly reduced(P<0.05),and the cell apoptosis rate was significantly increased(P<0.01)when compared with those in the blank group.The proliferation,migration,and invasion abilities of BHP-10-3 in the si-LINC00365+miR-519d inhibitor group were significantly increased(P<0.05),and the cell ap-optosis rate was significantly reduced(P<0.01)when compared with those in the si-LINC00365 group.Conclusion LINC00365 can target miR-519d to affect the proliferation,migration,inva-sion and apoptosis of thyroid cancer cells.关键词
LINC00365/甲状腺癌/增殖/凋亡/迁移/侵袭Key words
LINC00365/thyroid cancer/proliferation/apoptosis/migration/invasion分类
基础医学引用本文复制引用
吾甫尔·依马尔,王护国,达尔汗·赛力克别克,邓超,马依热·努尔买卖提..LINC00365靶向调控miR-519d影响甲状腺癌细胞的增殖、凋亡、迁移与侵袭[J].医学分子生物学杂志,2025,22(2):117-123,138,8.基金项目
新疆维吾尔自治区自然科学基金(No.2021D01C325) This work was supported by a grant from the Natural Science Foundation of Xinjiang Uygur Autonomous Region(No.2021D01C325) （No.2021D01C325）
