医学分子生物学杂志2025，Vol.22Issue(2)：124-130,7.DOI:10.3870/j.issn.1672-8009.2025.02.004
莲心碱通过ROS/JNK途径诱导人胶质瘤U251细胞的凋亡
Liensinine Induces Apoptosis in Glioma U251 Cells through ROS/JNK Pathway
摘要
Abstract
Objective To investigate the effect of liensinine(LI)on apoptosis of U251 glioma cells and its mechanisms.Methods U251 and HEB cells were treated with different concentrations of LI for 24/48 h,CCK8 assay and colony formation assay were performed to detect cell viability,flow cytometer was performed to detect apoptosis,and the expression levels of apoptotic proteins(Bax/Bcl-2,cleaved PARP)were detected by Western blotting.Cells were divided into 6 groups:control,LI,NAC,LI+NAC,SP600125,LI+SP600125.ROS was detected by DCFH-DA and p-JNK/cleaved PARP by Western blotting.U251 xenograft model was established to evaluate the effect of LI on tumor volume/weight and tumor cell apoptosis.Results LI significantly inhibited the proliferation of U251 cells,decreased the colony formation of U251 cells,and promoted apopto-sis.LI(80 μmol/L)induced ROS production and JNK phosphorylation,which were reversed by NAC and SP600125.In vivo experiments showed that LI inhibited tumor growth,reduced Ki67 posi-tive cells,and promoted apoptosis.Conclusion LI selectively inhibits U251 via ROS/JNK-media-ted apoptosis,demonstrating therapeutic potential for glioma.关键词
胶质瘤/莲心碱/细胞凋亡/自噬/LC3BKey words
glioma/liensinine/apoptosis/autophagy/LC3B分类
临床医学引用本文复制引用
李承科,何琴,罗孝全,冯浩,谯飞..莲心碱通过ROS/JNK途径诱导人胶质瘤U251细胞的凋亡[J].医学分子生物学杂志,2025,22(2):124-130,7.基金项目
四川省科技计划项目(No.2023JDRC0091) This work was supported by a grant from the Science and Technology Pro-ject of Sichuan Province(No.2023JDRC0091) （No.2023JDRC0091）
