医学分子生物学杂志2025，Vol.22Issue(2)：131-138,8.DOI:10.3870/j.issn.1672-8009.2025.02.005
基于高通量质谱技术对糖尿病伴行为学异常小鼠模型的多组学分析及验证
Multi-omics Analysis and Validation of a Mouse Model of Diabetes Mellitus with Behavioral Abnormalities Based on High-throughput Mass Spectrometry
摘要
Abstract
Objective To screen and verify the differentially expressed protein and the metabo-lites in a mouse model of type 2 diabetes mellitus with cognitive behavior abnormalities by high-throughput mass spectrometry.Methods High-throughput mass spectrometry was used to detect the levels of hippocampal tissue protein expression and serum metabolites in the DB group(abnormal cognitive behavior group)and the M group(normal cognitive behavior group).Target proteins and metabolites were screened out by bioinformatics and were then verified through Western blotting and ELISA.Results ①45 proteins and 119 positive ion-and 110 negative ion-metabolites were found to be significantly expressed,and KEGG analysis showed that the differentially expressed genes were enriched in multiple metabolic pathways.② Correlation analysis showed that serotonin-containing synapses and purine metabolism pathways were pathways co-enriched by multi-omics analysis.③pyruvate kinase L/R(PKLR)、arachidonic acid(AA)and adenosine 5′-monophosphate,(AMP)were differentially expressed.Conclusion PKLR,AA and AMP may be used as potential biomarkers for the diagnosis of type 2 diabetes mellitus with cognitive dysfunction.关键词
2型糖尿病/db/db小鼠/认知障碍/多组学Key words
type 2 diabetes/db/db mice/cognitive impairment/multi-omics分类
基础医学引用本文复制引用
杨淑芳,张浩强,陈茜,范琳,余烨璠,王少华..基于高通量质谱技术对糖尿病伴行为学异常小鼠模型的多组学分析及验证[J].医学分子生物学杂志,2025,22(2):131-138,8.基金项目
南京医科大学泰州临床医学院重点研究项(No.TZKY20230307),泰州市人民医院课题(No.ZL202219) This work was supported by grants from the Key Research Project of Taizhou Clinical Medical College of Nanjing Medical University(No.TZKY20230307),the Project of Taizhou People's Hospital(No.ZL202219) （No.TZKY20230307）
