医学分子生物学杂志2025，Vol.22Issue(2)：153-159,7.DOI:10.3870/j.issn.1672-8009.2025.02.008
EPB41通过介导脂肪酸氧化影响食管癌增殖和转移的机制研究
Effect of EPB41 Mediated Fatty Acid Oxidation on Esophageal Cancer Cell Proliferation and Migration
摘要
Abstract
Objective To investigate the effect of erythrocyte membrane protein band 4.1(EPB41)on the malignant phenotype of esophageal cancer cells and explore the possible underlying mechanisms based on fatty acid oxidation(FAO)mediated by the Wnt/β-catenin signaling path-way.Methods KYSE30 cells and KYSE150 cells in logarithmic growth phase were divided into 5 groups each.The mRNA and protein expression levels,cell proliferation and migration rate,ATP level,NADPH/NADP+ratio,reactive oxygen species(ROS)and GSH levels were detec-ted.Results In KYSE30 cells,the number of cell clones,cell migration rate,ATP level,NAD-PH/NADP+ratio,ROS level,and protein expression levels of Wnt3a,β-catenin,and nuclear β-catenin in cells were significantly increased in the EPB41 siRNA group(P<0.05),and fatty acid oxidation inhibitor and Wnt pathway inhibitor could reverse the effect of EPB41.In KYSE150 cells,the number of clones,cell migration rate,ATP level,NADPH/NADP+ratio,ROS level,and the expression levels of Wnt3a,β-catenin,and nuclear β-catenin proteins were significantly re-duced in the cells in the EPB41 overexpression group(P<0.05),and the effect of EPB41 could be reversed by the fatty acid oxidation inducer and the Wnt pathway activator.Conclusion EPB41 expression is down-regulated in esophageal cancer cells.EPB41 overexpression can inhibit the prolif-eration and migration of esophageal cancer cells by suppressing the FAO pathway,which may be a-chieved by modulating the Wnt/β-catenin signaling transduction.关键词
EPB41/食管癌/脂肪酸氧化/Wnt/β-catenin信号通路Key words
erythrocyte membrane protein band 4.1/esophageal cancer/fatty acid oxidation/Wnt/β-catenin signaling分类
基础医学引用本文复制引用
林近秋,王亮,刘石柱..EPB41通过介导脂肪酸氧化影响食管癌增殖和转移的机制研究[J].医学分子生物学杂志,2025,22(2):153-159,7.基金项目
2022 年河北省医学科学计划研究课题(No.20220386) This work was supported by a grant from the Hebei Medical Science Program in 2022(No.20220386) （No.20220386）
