医学分子生物学杂志2025，Vol.22Issue(2)：160-165,6.DOI:10.3870/j.issn.1672-8009.2025.02.009
干扰Rho相关卷曲螺旋形成蛋白激酶1表达对急性肺损伤小鼠的保护作用
Protective Effect of Interfering with Rho-associated Coil Formation Protein Kinase 1 Expression on Mice with Acute Lung Injury
摘要
Abstract
Objective To investigate the effect of interference with Rho-associated coil forming protein kinase 1 on lung injury induced by lipopolysaccharide(LPS)in mice.Methods C57BL/6 mice were divided into 4 groups:healthy control group,model group,negative control group and ROCK1 interference group,10 mice each group.Acute lung injury was induced with LPS(5 mg/kg,i.p.).The expression level of ROCK1 was detected by RT-PCR and Western blotting.The pul-monary function detection system was used to detect the airway resistance,resting ventilation and lung volume in mice.HE staining was used to observe the pathological damage of lung tissues,and Masson staining was used to observe the pulmonary fibrosis.Western blotting was used to detect the protein expression levels of α-SMA,TGF-β1 and FN.TUNEL assay was used to detect the lung cell apoptosis.The levels of iNOS,IL-6 and TNF-α were detected by ELISA.Results ROCK1 was highly expressed in the model group and the lung injury was more serious when compared with that in the healthy control group.ROCK1 interference group had lower expression level of ROCK1(P<0.05),and increased airway resistance,resting ventilation and lung volume when compared with the model group(P<0.05).The pathological injury and degree of fibrosis in lung tissues were im-proved in the ROCK1 interference group,and the expression levels of α-SMA,TGF-β1 and FN proteins were significantly decreased(P<0.05).The number of apoptotic cells in lung tissues was significantly decreased(P<0.05),and the levels of iNOS,IL-6 and TNF-α in peripheral blood were significantly decreased in the ROCK1 interference group when compared with those in the model group(P<0.05).Conclusion Interfering ROCK1 expression can improve lung function,pulmonary fibrosis,apoptosis and inflammation in LPS-induced acute lung injury mice.关键词
ROCK1/脂多糖/急性肺损伤Key words
ROCK1/lipopolysaccharide/acute lung injury分类
临床医学引用本文复制引用
李玉芳,杨楠,于小纬,魏志伟,左天昕,邹芳..干扰Rho相关卷曲螺旋形成蛋白激酶1表达对急性肺损伤小鼠的保护作用[J].医学分子生物学杂志,2025,22(2):160-165,6.基金项目
2023 年度河北省医学科学研究课题(No.20231415) This work was supported by a grant from the 2023 Hebei Province Medical Science Research Project(No.20231415) （No.20231415）
