医学分子生物学杂志2025，Vol.22Issue(2)：173-179,7.DOI:10.3870/j.issn.1672-8009.2025.02.011
miR-425-5p在子宫内膜癌中的诊断价值及其对子宫内膜癌细胞增殖、迁移和凋亡的影响
Diagnostic Value of miR-425-5p in Endometrial Cancer and Effect of Inhibiting miR-425-5p on Proliferation,Migration and Apoptosis in Endometrial Cancer Cells
摘要
Abstract
Objective To analyze the diagnostic value of miR-425-5p in endometrial cancer(EC)and the effect of inhibiting miR-425-5p on the proliferation,migration,and apoptosis of EC cells.Methods The expression level of miR-425-5p in serum and tissues of patients with EC and benign uterine lesions was detected by RT-qPCR,and was compared among patients with different pathological characteristics of EC.The relationship between miR-425-5p expression in the tissues and serum and the pathological characteristics was analyzed,as well as its diagnostic value for EC.The expression level of miR-425-5p in normal endometrial epithelial hEEC cells,endometrial carcinoma Ishikawa,HEC-1A,HEC-1B cells was detected by RT-qPCR.Ishikawa cells were divided into 3 groups:Con group(no treatment control),anti-miR-NC group(transfected with anti-miR-425-5p)and anti-miR-425-5p group(transfected with anti-miR-425-5p).RT-qPCR was used to detect the miR-425-5p expression level.MTT and flow cytometry were used to detect cell proliferation,cell cycle and apoptosis.cell migration was detected by Transwell.The expression levels of CyclinD1,PCNA,Bax,Bcl-2 and MMP9 were detected by Western blotting.Results The expression level of miR-425-5p in serum and tissues of the endometrial carcinoma group was higher than that of the be-nign lesion group(P<0.05).There were statistically significant differences when comparing the ex-pression level of miR-425-5p in patients with different lymph node metastasis statuses,FIGO stages and degrees of differentiation(P<0.05).The expression of miR-425-5p in tissues and serum was positively correlated with FIGO stage,lymph node metastasis,and degree of differentiation(r=0.793,0.689,0.665,0.694,0.652,0.672,P<0.05).The AUC of miR-425-5p in tissues and serum for the diagnosis of EC were 0.782 and 0.762,respectively.The sensitivities were 74.36%,71.79%,specificity,and the specificities were 71.79%,70.51%,respective-ly.The expression level of miR-425-5p in endometrial carcinoma Ishikawa,HEC-1A,HEC-1B cells was increased when compared with that in hEEC cells(P<0.05).The expression level of miR-425-5p,cell activity,the proportion of cells in the S phase,the number of migrated cells,and the expression levels of Bcl-2,MMP9,CyclinD1 and PCNA proteins in the anti-miR-425-5p group were significantly decreased when compared with those in the anti-miR-NC group or the Con group,while the proportion of cells in the G0/G1 phase,the apoptosis rate and the protein expres-sion of Bax were significantly increased(P<0.05).Conclusion miR-425-5p is highly expressed in EC tissues and serum,its expression level in EC tissues and serum is related to the patients'FI-GO stage,lymph node metastasis and degree of differentiation.It has certain values in the diagnosis of EC.Moreover,inhibition of miR-425-5p can significantly inhibit the proliferation and migration of EC cells,and induce cell apoptosis.关键词
miR-425-5p/子宫内膜癌/诊断/细胞增殖/细胞周期/细胞凋亡Key words
miR-425-5p/endometrial cancer/diagnosis/cell proliferation/cell cycle/ap-optosis分类
医药卫生引用本文复制引用
白咪红,张洁,寇莉,王金波..miR-425-5p在子宫内膜癌中的诊断价值及其对子宫内膜癌细胞增殖、迁移和凋亡的影响[J].医学分子生物学杂志,2025,22(2):173-179,7.基金项目
宝鸡市卫生健康科研项目(No.2023-096) This work was supported by a grant from Baoji Health Research Project(No.2023-096) （No.2023-096）
