医学分子生物学杂志2025，Vol.22Issue(2)：194-199,6.DOI:10.3870/j.issn.1672-8009.2025.02.014
miR-495-3p及Mn-SOD基因检测在糖尿病肾病患者疾病评估中的应用研究
Application of miR-495-3p and Mn-SOD Gene Detection in Disease E-valuation of Diabetes Nephropathy Patients
朱双 1胡晓1
作者信息
- 1. 黄石市中心医院 湖北省 黄石市,435000
- 折叠
摘要
Abstract
Objective To explore the application value of gene detection of miR-495-3p and manganese superoxide dismutase(Mn-SOD)in the disease evaluation of diabetes nephropathy pa-tients.Methods A total of 150 patients with diabetes nephropathy who were treated in Huangshi Central Hospital from January 2022 to December 2023 were selected,and another 150 patients with diabetes and 150 healthy people were included in this study as controls.the differences of miR-495-3p expression and Mn-SOD genotypes in each group were compared,and the value of miR-495-3p expression and Mn-SOD genotypes in predicting poor prognosis of diabetes nephropathy were ana-lyzed.Results The expression level of miR-495-3p in the group of diabetes nephropathy patients was(0.56±0.14),which was significantly lower than that of diabetes and healthy people(P<0.05).There was a statistically significant difference in the distribution of genotypes at the 9 and 5 777 loci of Mn-SOD gene in diabetes nephropathy,diabetes and healthy people(P<0.05).The proportion of CT+CC genotype at the 9 loci of Mn-SOD gene in diabetes nephropathy was 40.00%,the proportion of CT+CC genotype at position 5 777 of the Mn-SOD gene was 98.00%.The expres-sion level of miR-495-3p in diabetes nephropathy patients with T2DM duration≥10 years was(0.46±0.14),which was significantly lower than that in patients with T2DM duration<10 years(P<0.05).The expression level of miR-495-3p in patients with poor prognosis of diabetes nephrop-athy was(0.47±0.14),which was significantly lower than that in patients with good prognosis(P<0.05).The proportion of CT+CC at Mn-SOD gene locus 9 genotype in patients with poor prog-nosis was 73.33%,which was significantly higher than that in patients with good prognosis(P<0.05).There was no significant difference in CT+CC ratio of Mn-SOD gene 5 777 genotype between patients with poor prognosis and those with good prognosis(P>0.05).The area under the ROC curve for predicting poor prognosis of miR-495-3p and Mn-SOD gene 9 locus genotypes was 0.784(95%CI:0.705-0.863)and 0.786(95%CI:0.700-0.872),respectively(P<0.05).Conclusion The level of miR-495-3p in patients with diabetes nephropathy is reduced,miR-495-3p expression and Mn-SOD genetypes have certain application values in predicting the prognosis of diabetes nephropathy.关键词
miR-495-3p/锰超氧化物歧化酶/糖尿病肾病/预后Key words
miR-495-3p/manganese superoxide dismutase/diabetes nephropathy/prognosis分类
医药卫生引用本文复制引用
朱双,胡晓..miR-495-3p及Mn-SOD基因检测在糖尿病肾病患者疾病评估中的应用研究[J].医学分子生物学杂志,2025,22(2):194-199,6.
