南方农业学报2025，Vol.56Issue(2)：378-391,14.DOI:10.3969/j.issn.2095-1191.2025.02.004
文冠果HD-Zip基因家族成员鉴定及其对盐胁迫的响应
Identification of HD-Zip gene family members in Xanthoceras sorbifolium Bunge and their response to salt stress
摘要
Abstract
[Objective]The members of homedomain-leucine zipper(HD-Zip)gene family in Xanthoceras sorbifo-lium Bunge were identified,and their expression under salt stress conditions was analyzed to provide theoretical basis for mining salt tolerant genes in X.sorbifolium and for breeding new salt tolerant varieties.[Method]Based on the whole-genome data of X.sorbifolium,the members of the HD-Zip gene family were identified.The physicochemical properties,conserved motifs,gene structures,cis-acting elements,and phylogenetic evolutionary relationships of the HD-Zip gene family were predicted and analyzed using bioinformatics tools.Subcellular localization was performed by double enzyme digestion.Two-month-old X.sorbifolium seedlings were subjected to salt stress treatments with NaCl concentrations of 50,100,and 150 mmol/L,with a control group(CK)treated with 0 mmol/L NaCl.The tissue expression patterns of HD-Zip family genes under salt stress in roots and leaves of X.sorbifolium were analyzed using real-time fluorescence quanti-tative PCR.[Result]A total of 29 HD-Zip gene family members were identified from the full genome of X.sorbifolium,named XsHB1-XsHB29.Except for XsHB29,XsHB1-XsHB28 genes were all located on the 12 chromosomes of X.sorbifo-lium.The number of amino acids encoded by HD-Zip family members ranged from 202 to 838,with molecular weights ranging from 23567.07 to 92421.90 kD,and theoretical isoelectric points(pI)ranging from 4.75 to 8.98.Subcellular lo-calization results showed that proteins of the family were located in the nucleus.Phylogenetic analysis divided the 29 HD-Zip gene family members into 4 subfamilies(I,II,III,IV),and the genes and proteins of each subfamily member had similarities in structure.Through the prediction of cis-acting elements in the promoter,it was found that the pro-moters of HD-Zip genes family had 22 types of cis-acting elements,such as abiotic stress and plant hormone response ele-ments.Real-time fluorescence quantitative PCR results showed that the relative expression levels of XsHB1,XsHB12,XsHB13 and XsHB29 genes in leaves treated with 50 mmol/L NaCl were significantly up-regulated(P<0.05,the same below);while the relative expression levels of XsHB5,XsHB12,XsHB13,XsHB24 and XsHB29 genes were signifi-cantly down-regulated after treatment with 150 mmol/L NaCl.Subcellular localization and transient transformation results showed that XsHB5 was a nuclear-localized protein and could negatively regulate the salt tolerance of X.sorbifolium.[Conclusion]A total of 29 members of XsHD-Zip gene family can be screened from X.sorbifolium,they are divided into 4 subfamilies,and the members of each subfamily have similar gene and protein structure.Among them,genes such as XsHB1,XsHB5,XsHB12,XsHB13,XsHB24 and XsHB29 may play important roles in the salt stress response of X.sorbi-folium.The HD-Zip gene family is involved in regulating the growth,development,metabolism,and other emergency re-sponse processes of X.sorbifolia.关键词
文冠果/HD-Zip基因家族/盐胁迫/基因表达Key words
Xanthoceras sorbifolium Bunge/HD-Zip gene family/salt stress/gene expression分类
农业科技引用本文复制引用
朱华利,陈玉霞,钱程,陈玉金,于涵,姜涛,李璐璐,鲁仪增..文冠果HD-Zip基因家族成员鉴定及其对盐胁迫的响应[J].南方农业学报,2025,56(2):378-391,14.基金项目
国家自然科学基金项目(32201611) （32201611）
山东省自然资源厅"珍贵树种保育利用创新团队"项目(鲁自然字[2023]98号) （鲁自然字[2023]98号）
济南百合园林集团有限公司科技创新合作项目(BHYLJY-KT-24-0102) National Natural Science Foundation of China(32201611) （BHYLJY-KT-24-0102）
Shandong Department of Natural Re-sources"Precious Tree Species Conservation and Utilization Innovation Team"Project(Luziranzi[2023]98) （Luziranzi[2023]98）
Scientific and Technological Innovation Cooperation Project of Jinan Baihe Gardening Group Co.,Ltd.(BHYLJY-KT-24-0102) （BHYLJY-KT-24-0102）
