南方农业学报2025，Vol.56Issue(2)：392-406,15.DOI:10.3969/j.issn.2095-1191.2025.02.005
去顶诱导滇红月季腋芽萌发过程中的转录组分析
Transcriptome analysis during the sprouting of axillary buds in-duced by topping in Rosa chinensis'Dianhong'
摘要
Abstract
[Method]This study aimed to identify candidate genes involved in axillary buds sprouting of roses and to elucidate the molecular mechanism of which strigolactolides(SLs)regulated this process,thereby establishing a theoreti-cal foundation for the regulation of plant archeitecture control in roses.[Method]Using the low-branching rose variety,Rosa chinensis'Dianhong',as experimental material,samples of the first axillary buds and root tips were collected be-fore topping(0 h)and at 12,24 and 48 h after topping for transcriptome sequencing.Differentially expressed genes(DEGs)were screened from the first axillary bud samples before and after topping,root tip samples before and after top-ping,as well as from the first axillary bud and root tip samples at the same time point,using the criteria of|log2 Fold Change|≥1 and P<0.05.The DEGs were subjected to GO functional annotation and KEGG pathway enrichment analysis to identify genes involved in the biosynthesis and signaling pathways of strigolactones(SLs)that regulated the outgrowth of rose axillary buds.These findings were further validated by real-time fluorescence quantitative PCR.[Result]Follo-wing topping,the length of the first axillary bud exhibited an increasing trend over time.The number of DEGs in both the first axillary bud and root tip peaked at 12 h after topping,followed by 24 h after topping,and was the lowest at 48 h after topping.A total of 5377 shared DEGs were identified in both the first axillary bud and root tip at the same time points of topping.GO functional annotation and enrichment analysis revealed that 721 DEGs were enriched in the oxidoreductase activity category,while 698 DEGs were enriched in the oxidation-reduction process.KEGG pathway enrichment analy-sis indicated that the DEGs were significantly enriched in pathways related to phenylpropanoid biosynthesis,plant-pathogen interaction,plant hormone signal transduction,starch and sucrose metabolism.A total of 13 related genes linked to SLs biosynthesis and signaling transduction pathway were identified,among them,D27(Chr3g0487411),D27(Chr6g0289711),D27(Chr7g0187341),MAX1,CCD7 and CCD8B were related to biosynthesis,and SMXL7,SMXL8,DAD2,TB1/BRC1,MAX2A,D14(Chr2g0091021),D14(Chr3g0478651)were related to SLs transduction pathway.Results of real-time fluorescence quantitative PCR confirmed the reliability of transcriptome data.[Conclusion]Topping promotes axillary bud sprouting and induces the differential gene expression in the first axillary buds and root tips.Axil-lary bud sprouting is hypothesized to be regulated by plant hormone signaling transduction and carbohydrate metabo-lism.The 13 identified SLs biosynthesis and signaling transduction-related genes may be candidate genes involved in axil-lary bud sprouting of rose.关键词
月季/去顶处理/腋芽萌发/转录组/差异表达基因Key words
rose/topping treatment/axillary bud sprouting/transcriptome/differentially expressed gene分类
农业科技引用本文复制引用
管嵩,朱思雅,罗中元,贺水莲,赵雁,孟静..去顶诱导滇红月季腋芽萌发过程中的转录组分析[J].南方农业学报,2025,56(2):392-406,15.基金项目
国家自然科学基金项目(31560227) （31560227）
云南省基础研究计划项目(202201AT070256) （202201AT070256）
云南省农业联合专项(202301BD070001-032) National Natural Science Foundation of China(31560227) （202301BD070001-032）
Yunnan Basic Research Plan Project(202201AT070256) （202201AT070256）
Yunnan Agricultural Joint Special Project(202301BD070001-032) （202301BD070001-032）
