南方农业学报2025，Vol.56Issue(2)：441-451,11.DOI:10.3969/j.issn.2095-1191.2025.02.009
文冠果TCP基因家族鉴定及盐胁迫下表达分析
Identification of TCP gene family in Xanthoceras sorbifolium and expression analysis under salt stress
摘要
Abstract
[Objective]The TCP gene family of Xanthoceras sorbifolium was identified and its expression under salt stress was analyzed,its response mechanism to salt stress was studied,which provided theoretical basis for breeding of salt-tolerant germplasm of X.sorbifolium.[Method]The TCP gene family of X.sorbifolium was identified by bioinforma-tics method,and the protein physicochemical properties,phylogenetic evolution,conserved motifs,gene structure,chromo-some localization and promoter cis-acting elements were analyzed.Real-time fluorescence quantitative PCR was used to detect the expression of TCP genes of X.sorbifolium(XsTCPs)in leaf and root under salt stress.[Result]A total of 24 members of the TCP gene family(XsTCP1-XsTCP24)of X.sorbifolium were identified,encoding 185-576 amino acids residues,protein molecular weight being 20576.21-63366.92 kD,theoretical isoelectric point being 4.62-10.25,all of which were hydrophilic proteins and located in the nucleus.Based on the phylogenetic analysis results,the 24 XsTCPs proteins could be divided into 3 subfamilies,among which the PCF subfamily contained 14 members,the CIN subfamily contained 6 members,and the CYC/TB1 subfamily contained 4 members.Except that XsTCP23 and XsTCP24 genes were unable to locate on the chromosome,the remaining TCP gene family members were unevenly distributed on 11 chromo-somes in the form of single genes or gene clusters.A total of 10 conserved motifs were found in 24 XsTCPs proteins,among which Motif 1 was common to all proteins.The TCP gene family members of X.sorbifolium contained light response elements,hormone response elements,stress response elements and plant growth and development elements.XsTCP4,XsTCP6,XsTCP7,XsTCP10,XsTCP12,XsTCP13,XsTCP21 and XsTCP22 genes contained drought-induced MYB binding elements.The results of real-time fluorescence quantitative PCR showed that the expression of the above genes changed greatly in the leaves and roots of X.sorbifolium under salt stress,suggesting that the above 8 XsTCPs genes were involved in the process of salt stress response.[Conclusion]XsTCPs proteins may be involved in biological pro-cesses by regulating various target genes involved in growth,development,metabolism and stress response.It is specu-lated that 8 XsTCPs genes containing drought-induced MYB binding elements may help improve the resistance to salt stress.关键词
文冠果/TCP基因家族/盐胁迫/表达分析Key words
Xanthoceras sorbifolium/TCP gene family/salt stress/expression analysis分类
农业科技引用本文复制引用
陈玉金,于涵,朱华利,姜涛,刘博,李伟,赵永军,鲁仪增,解孝满..文冠果TCP基因家族鉴定及盐胁迫下表达分析[J].南方农业学报,2025,56(2):441-451,11.基金项目
山东省重点研发计划(农业良种工程)项目(2020LZGC0904) （农业良种工程）
山东省自然资源厅珍贵树种保育利用创新团队项目(鲁自然资字[2023]98号) Shandong Key Research and Development Plan(Agricultural Elite Variety Project)Project(2020LZGC0904) （鲁自然资字[2023]98号）
Innovation Team of Rare Tree Species Conservation and Utilization Project of Shandong Natural Resources Department(LZRZZ[2023]98) （LZRZZ[2023]98）
