南方农业学报2025，Vol.56Issue(2)：485-495,11.DOI:10.3969/j.issn.2095-1191.2025.02.013
不同石斛生态位差异和分子特征分析
Ecological niche differences and molecular characteristics of different Dendrobium
摘要
Abstract
[Objective]By analyzing the ecological niche differences and molecular characteristics of different Dendro-bium species,the genetic and ecological relationships among different Dendrobium species were revealed,which provided scientific basis for the protection and utilization of Dendrobium wild resources.[Method]Three representative Dendro-bium(D.huoshanense,D.officinale and D.moniliforme)were selected as research subjects.Species occurrence data for these Dendrobium species in 3 major distribution areas in China were obtained through a combination of database informa-tion and field surveys.Data on 19 ecological factors were retrieved from the WorldClim database.The correlations among these ecological factors were analyzed,and the characteristic niches of the 3 Dendrobium species were calculated.The internal transcribed spacer(ITS)sequences of the 3 Dendrobium species were amplified and sequenced using PCR.Based on the similarity of the ITS sequences of the 3 Dendrobium species,a phylogenetic tree was constructed using the Neighbor-joining method to analyze their phylogenetic relationships.[Result]The correlation analysis of ecological factors revealed that the 7 major ecological factors were annual mean temperature,monthly mean diurnal temperature range,isothermality,annual temperature range,precipitation of the driest season,and precipitation of the warmest season.The niche overlap between D.huoshanense and the other 2 species,D.officinale and D.moniliforme,was relatively low,at 0.95%and 1.87%respectively,indicating that D.huoshanense had a specific ecological niche requirement.In contrast,the niche overlap between D.officinale and D.moniliforme was high,reaching 51.40%.When the 3 Dendrobium species were compared in pairwise,the null hypothesis of niche equivalence was not rejected(P>0.05).The 3 Dendrobium spe-cies had different ecological niches at present,the possibility that they occupied similar environmental niches could not be ruled out,which indicated that the mixed cultivation of the 3 Dendrobium species in the field was feasible.The overlap of D.officinale and D.moniliforme was the most,which indicated that the environmental conditions or resource sharing de-gree of the two was the highest,and the competition was the most intense.The length of ITS sequence of the 3 Dendro-bium species ranged from 690 to 710 bp.Phylogenetic tree results showed that species alone formed 1 highly supported group,while the individuals of D.moniliforme and D.huoshanense were grouped into 2 other groups with low support.[Conclusion]Compared with D.officinale and D.moniliforme,D.huoshanense has more specific requirements for eco-logical factors.D.huoshanense and D.moniliforme are more closely related.ITS sequence analysis can distinguish D.offi-cinale from D.moniliforme and D.huoshanense.关键词
石斛/生态位差异/ITS序列/分子鉴定Key words
Dendrobium/ecological niche differences/ITS sequences/molecular identification分类
农业科技引用本文复制引用
周润洋,刘思安..不同石斛生态位差异和分子特征分析[J].南方农业学报,2025,56(2):485-495,11.基金项目
国家自然科学基金项目(32001341) National Natural Science Foundation of China(32001341) （32001341）
